Nucleolin antibody

Mouse anti Nucleolin

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Nucleolin

Product Code Applications Pack Size List Price Your Price Qty
VMA00665
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human nucleolin antibody recognizes nucleolin.

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20 (provided by RefSeq, Jul 2008).

Mouse anti Human nucleolin antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti nucleolin antibody detects a band of approximately 100 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Lysate of SU-DHL-1 nuclei
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19338
Entrez Gene
NCL
GO Terms
GO:0000166 nucleotide binding
GO:0001525 angiogenesis
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005730 nucleolus
GO:0005938 cell cortex
GO:0042162 telomeric DNA binding
GO:0008022 protein C-terminus binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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