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Nucleolin antibody

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Western Blot Validated
Anti Nucleolin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with nucleolin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to nucleolin (molecular weight 100 kDa).

Mouse anti Nucleolin

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00665
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human nucleolin antibody recognizes nucleolin.

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20 (provided by RefSeq, Jul 2008).

Mouse anti Human nucleolin antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti nucleolin antibody detects a band of approximately 100 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Lysate of SU-DHL-1 nuclei

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
P19338 Related reagents
Entrez Gene
NCL Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0001525 angiogenesis
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005730 nucleolus
GO:0005938 cell cortex
GO:0042162 telomeric DNA binding
GO:0008022 protein C-terminus binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Nucleolin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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Request a different product with the Nucleolin specificity