Nuclear Antigen antibody | 58-15
Mouse anti Human nuclear antigen, clone 58-15 recognizes nuclear ribonucleoprotein particles in human cells. Ribonucleoprotein (RNP) is a nucleoprotein that contains RNA, i.e. it is an association that combines ribonucleic acid and protein together. A few examples are known like the ribosome, the enzyme telomerase and small nuclear RNPs (snRNPs).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig fraction - liquid
- Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3)
- Nuclear material from lymphoma cells.
- Molecular Weight
- 28 kD
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
Applications of Nuclear Antigen antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/20||1/30|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Human anti Mouse IgM:FITC||HCA040F||0.1 mg||F|
|Human anti Mouse IgM:HRP||HCA040P||0.1 mg||E|
|Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)||STAR138A||1 ml||C E P WB|
|Goat anti Mouse IgM:RPE||STAR86PE||0.5 ml||F|
|Goat anti Mouse IgG/A/M:Alk. Phos.||STAR87A||1 mg||C E WB|
|Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)||STAR87P||1 mg||E|
Product Specific References
References for Nuclear Antigen antibody
Clevenger, C. V. et al. (1985) A method for simultaneous nuclear immunofluorescence and DNA content quantitation using monoclonal antibodies and flow cytometry.
Cytometry 6(3): 208-214.