NUB1 antibody | OTI4H2

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Mouse anti NUB1

Mouse anti human NUB1 antibody recognizes NEDD8 ultimate buster 1, also known as negative regulator of ubiquitin-like proteins 1, BS4, renal carcinoma antigen NY-REN-18 or NYREN18. This antibody has been extensively validated for western blotting using whole cell lysates.

NUB1 Antibody with Control Lysate

Mouse anti human NUB1 antibody recognizes NEDD8 ultimate buster 1, also known as negative regulator of ubiquitin-like proteins 1, BS4, renal carcinoma antigen NY-REN-18 or NYREN18. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4H2
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00450 WB 100 µl
VMA00450KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human NUB1 antibody recognizes the NUB1, also known as negative regulator of ubiquitin-like proteins 1, NEDD8 ultimate buster-1, BS4, renal carcinoma antigen NY-REN-18 or simply NYREN18, .

The NUB1 gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by NUB1 regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of NUB1 results in multiple transcript variants (provided by RefSeq, Aug 2011).

Mouse anti Human NUB1 antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti NUB1 detects a band of approximately 78 kDa in Raji cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-326 of human NUB1 (NP_057202) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y5A7 Related reagents
Entrez Gene
NUB1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016567 protein ubiquitination
GO:0005634 nucleus
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0034341 response to interferon-gamma
GO:0034612 response to tumor necrosis factor
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NUB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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