NR2C2 antibody | OTI1E1

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Mouse anti NR2C2

NR2C2 antibody recognizes nuclear receptor subfamily 2 group C member 2, also known as nuclear hormone receptor TR4, orphan nuclear receptor TAK1, orphan nuclear receptor TR4 or testicular nuclear receptor 4. This antibody has been extensively validated for western blotting using whole cell lysates.

NR2C2 Antibody with Control Lysate

NR2C2 antibody recognizes nuclear receptor subfamily 2 group C member 2, also known as nuclear hormone receptor TR4, orphan nuclear receptor TAK1, orphan nuclear receptor TR4 or testicular nuclear receptor 4. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1E1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00214 WB 100 µl
VMA00214KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human NR2C2 antibody recognizes the nuclear receptor subfamily 2 group C member 2, also known as nuclear hormone receptor TR4, nuclear receptor subfamily 2 group C member 2, orphan nuclear receptor TAK1, orphan nuclear receptor TR4 and testicular nuclear receptor 4.

Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996. PubMed 8661150) (supplied by OMIM, Mar 2008).

Mouse anti Human NR2C2 antibody detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-241 of human NR2C2 (NP_003289) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P49116 Related reagents
Entrez Gene
NR2C2 Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0003713 transcription coactivator activity
GO:0005654 nucleoplasm
GO:0007283 spermatogenesis
GO:0007399 nervous system development
GO:0008270 zinc ion binding
GO:0030154 cell differentiation
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0043565 sequence-specific DNA binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NR2C2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti NR2C2 detects a band of approximately 67 kDa in Jurkat cell lysates
Western Blotting
Anti NR2C2 detects a band of approximately 67 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB