NR2C2 antibody | OTI1E1

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Anti NR2C2 Antibody, clone OTI1E1 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti NR2C2 Antibody, clone OTI1E1 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human NR2C2 antibody, clone OTI1E1 (**VMA00214**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to NR2C2 (molecular weight 67 kDa).

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Mouse anti NR2C2

Product Type: PrecisionAb Monoclonal
Clone: OTI1E1
Isotype: IgG2a
Format: Purified
Specificity: NR2C2

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00214 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human NR2C2 antibody, clone OTI1E1 recognizes the nuclear receptor subfamily 2 group C member 2, also known as nuclear hormone receptor TR4, nuclear receptor subfamily 2 group C member 2, orphan nuclear receptor TAK1, orphan nuclear receptor TR4 and testicular nuclear receptor 4.

Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al. 1996).

Mouse anti Human NR2C2 antibody, clone OTI1E1 detects a band of 67 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti NR2C2 detects a band of approximately 67 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-241 of human NR2C2 (NP_003289) produced in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P49116
Entrez Gene: NR2C2
GO Terms: GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003707 steroid hormone receptor activity; GO:0003713 transcription coactivator activity; GO:0005654 nucleoplasm; GO:0007283 spermatogenesis; GO:0007399 nervous system development; GO:0008270 zinc ion binding; GO:0030154 cell differentiation; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; View More GO Terms; GO:0043565 sequence-specific DNA binding; GO:0045944 positive regulation of transcription from RNA polymerase II promoter