NR1H4 antibody | CD01/3F5

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Mouse anti NR1H4

Product Type
PrecisionAb Monoclonal
Clone
CD01/3F5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00688
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human NR1H4 antibody recognizes NR1H4, also known as RXR-interacting protein 14, FXR, farnesoid X nuclear receptor, farnesol receptor HRR-1 and HRR1.

NR1H4 is a nuclear hormone receptor activated by bile acid and expressed along the length of the gastrointestinal tract (Cariello et al. 2018). NR1H4 is a transcription factor, considered to be a master regulator of synthesis and secretion of bile acid, as well as metabolism of lipids and glucose in the liver and intestine. Furthermore, NR1H4 plays a crucial role in the regulation of inflammatory responses in the intestinal tract, as well as liver inflammation and regeneration. Alterations in NR1H4 play a role in the pathogenicity of gastrointestinal tract diseases including inflammatory bowel disease, colorectal cancer and type II diabetes (Ding et al. 2015). NR1H4 functions as a tumor suppressor and has been suggested as a potential therapeutic target in cancer. As such, synthetic bile acid derivatives have been developed to activate NR1H4 (Labiano et al. 2018), and antifibrotic potential has been demonstrated in animal models (Verbeke et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti NR1H4 detects a band of approximately 52 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-486 of human NR1H4
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q96RI1
Entrez Gene
NR1H4
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0004887 thyroid hormone receptor activity
GO:0005654 nucleoplasm
GO:0008206 bile acid metabolic process
GO:0008270 zinc ion binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0043565 sequence-specific DNA binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NR1H4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml