NQO2 antibody | OTI1G2
Mouse anti NQO2
NQO2 antibody recognizes NQO2, also known as NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2, NRH:quinone oxidoreductase 2 or quinone reductase 2.
NQO2 Antibody with Control Lysate
NQO2 antibody recognizes NQO2, also known as NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2, NRH:quinone oxidoreductase 2 or quinone reductase 2.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1G2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00715 | WB | 100 µl | ||||
| VMA00715KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human NQO2 antibody recognizes NQO2, also known as NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2, NRH:quinone oxidoreductase 2, quinone reductase 2 and ribosyldihydronicotinamide dehydrogenase [quinone].
NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 (PubMed 9367528)) (supplied by OMIM, Jul 2010).
Mouse anti Human NQO2 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 (PubMed 9367528)) (supplied by OMIM, Jul 2010).
Mouse anti Human NQO2 antibody detects a band of 26 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti NQO2 detects a band of approximately 26 kDa in HeLa cell lysates
- Product Form
- Purified IgG - Liquid
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody affinity purified on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Immunogen
- HEK293-derived full length recombinant protein of human NQO2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P16083 Related reagents
- Entrez Gene
- NQO2 Related reagents
- GO Terms
- GO:0009055 electron carrier activity
- GO:0001512 dihydronicotinamide riboside quinone reductase activity
- GO:0046872 metal ion binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0055114 oxidation-reduction process
- GO:0008753 NADPH dehydrogenase (quinone) activity
- GO:0050662 coenzyme binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NQO2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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