NQO1 antibody

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Mouse anti NQO1

NQO1 antibody recognizes NAD(P)H dehydrogenase [quinone] 1, also known as azoreductase, DT-diaphorase, DTD, menadione reductase, NAD(P)H:quinone oxidoreductase 1, phylloquinone reductase, or quinone reductase 1. The antibody has been validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00039
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1 also known as NQO1, azoreductase, phylloquinone reductase or QR1. NQO1 is a cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.

NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C, diaziquone and deoxynyboquinone has become a focus area for chemotherapeutic studies (Parkinson, E. et al. 2013). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). NQO1 expression is significantly upregulated in hypertrophic astrocytes and myelin-laden macrophages of active and chronic active multiple sclerosis patients (van Horssen et al. 2006).

Mouse anti Human NQO1 antibody recognizes human NQO1 as a single band of ~32 kDa in HeLa cell line lysates under reducing conditions and detects endogenous levels of total NQO1 but does not cross-react with NQO2.

Product Details

Target Species
Human
Western Blotting
Anti NQO1 antibody detects a band of approximately 32 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human NQO1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P15559 Related reagents
Entrez Gene
NQO1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0003955 NAD(P)H dehydrogenase (quinone) activity
GO:0004128 cytochrome-b5 reductase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006805 xenobiotic metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0007271 synaptic transmission, cholinergic
GO:0009636 response to toxin
GO:0050662 coenzyme binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NQO1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
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