Mouse anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1 also known as NQO1, azoreductase, phylloquinone reductase or QR1. NQO1 is a cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.

NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C, diaziquone and deoxynyboquinone has become a focus area for chemotherapeutic studies (Parkinson, E. et al. 2013). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 ^*2 allele (Nerbert, D.W. et al. 2002). NQO1 expression is significantly upregulated in hypertrophic astrocytes and myelin-laden macrophages of active and chronic active multiple sclerosis patients (van Horssen et al. 2006).

Mouse anti Human NQO1 antibody recognizes human NQO1 as a single band of ~32 kDa in HeLa cell line lysates under reducing conditions and detects endogenous levels of total NQO1 but does not cross-react with NQO2.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000