NQO1 antibody
Mouse anti NQO1
NQO1 antibody recognizes NAD(P)H dehydrogenase [quinone] 1, also known as azoreductase, DT-diaphorase, DTD, menadione reductase, NAD(P)H:quinone oxidoreductase 1, phylloquinone reductase, or quinone reductase 1. The antibody has been validated for western blotting using whole cell lysates.
NQO1 Antibody with Control Lysate
NQO1 antibody recognizes NAD(P)H dehydrogenase [quinone] 1, also known as azoreductase, DT-diaphorase, DTD, menadione reductase, NAD(P)H:quinone oxidoreductase 1, phylloquinone reductase, quinone reductase 1 or QR1.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00039 | WB | 100 µl | ||||
| VMA00039KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1 also known as NQO1, azoreductase, phylloquinone reductase or QR1. NQO1 is a cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.
NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C, diaziquone and deoxynyboquinone has become a focus area for chemotherapeutic studies (Parkinson, E. et al. 2013). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). NQO1 expression is significantly upregulated in hypertrophic astrocytes and myelin-laden macrophages of active and chronic active multiple sclerosis patients (van Horssen et al. 2006).
Mouse anti Human NQO1 antibody recognizes human NQO1 as a single band of ~32 kDa in HeLa cell line lysates under reducing conditions and detects endogenous levels of total NQO1 but does not cross-react with NQO2.
NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C, diaziquone and deoxynyboquinone has become a focus area for chemotherapeutic studies (Parkinson, E. et al. 2013). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). NQO1 expression is significantly upregulated in hypertrophic astrocytes and myelin-laden macrophages of active and chronic active multiple sclerosis patients (van Horssen et al. 2006).
Mouse anti Human NQO1 antibody recognizes human NQO1 as a single band of ~32 kDa in HeLa cell line lysates under reducing conditions and detects endogenous levels of total NQO1 but does not cross-react with NQO2.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NQO1 antibody detects a band of approximately 32 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human NQO1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P15559 Related reagents
- Entrez Gene
- NQO1 Related reagents
- GO Terms
- GO:0009055 electron carrier activity
- GO:0003955 NAD(P)H dehydrogenase (quinone) activity
- GO:0004128 cytochrome-b5 reductase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006805 xenobiotic metabolic process
- GO:0006809 nitric oxide biosynthetic process
- GO:0007271 synaptic transmission, cholinergic
- GO:0009636 response to toxin
- GO:0050662 coenzyme binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of NQO1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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