NQO1 antibody | A180

100% Secure

Mouse anti NQO1

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1 also known as NQO1, azoreductase, phylloquinone reductase or QR1. NQO1 is a cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.

NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C, diaziquone and deoxynyboquinone has become a focus area for chemotherapeutic studies (Parkinson, E. et al. 2013). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). NQO1 expression is significantly upregulated in hypertrophic astrocytes and myelin-laden macrophages of active and chronic active multiple sclerosis patients (van Horssen et al. 2006).

Mouse anti Human NQO1 antibody recognizes human NQO1 as a single band of ~32 kDa in HeLa cell line lysates under reducing conditions and detects endogenous levels of total NQO1 but does not cross-react with NQO2.

Product Details

Target Species
Western Blotting
Anti NQO1 antibody detects a band of approximately 32 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Recombinant human NQO1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0009055 electron carrier activity
GO:0003955 NAD(P)H dehydrogenase (quinone) activity
GO:0004128 cytochrome-b5 reductase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006805 xenobiotic metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0007271 synaptic transmission, cholinergic
GO:0009636 response to toxin
GO:0050662 coenzyme binding
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of NQO1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra