NQO1 antibody | A180
NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert et al. 2002). Mouse anti human NQO1 antibody, clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Sato et al. 2010 and Fegerholm et al. 2008).
Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegal et al. 1998).
Mouse anti human NQO1 antibody, clone A180 is reported as suitable for use in immunofluorescence studies (Siegal et al. 2012).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat Weak
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Purified recombinant human NQO1
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- P15559 Related reagents
- Entrez Gene
- NQO1 Related reagents
- GO Terms
- GO:0009055 electron carrier activity
- GO:0003955 NAD(P)H dehydrogenase (quinone) activity
- GO:0004128 cytochrome-b5 reductase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006521 regulation of cellular amino acid metabolic process
- GO:0006805 xenobiotic metabolic process
- GO:0006809 nitric oxide biosynthetic process
- GO:0007271 synaptic transmission, cholinergic
- GO:0009636 response to toxin
- GO:0050662 coenzyme binding
- For research purposes only
Applications of NQO1 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human lung
- Western Blotting
- MCA2880GA detects a band of approximately 31kDa in HeLa and HepG2 cell lysates.
Secondary Antibodies Available
Product Specific References
References for NQO1 antibody
Siegel, D. et al. (1998) Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors.
Clin Cancer Res. 4 (9): 2065-70.
Anwar, A. et al. (2002) Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1.
J Biol Chem. 277 (16): 14060-7.
Anwar, A. et al. (2003) Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems.
J Biol Chem. 278: 10368-73.
Moran, J.L. et al. (1999) A potential mechanism underlying the increased susceptibility of individuals with a polymorphism in NAD(P)H:quinone oxidoreductase 1 (NQO1) to benzene toxicity.
Proc Natl Acad Sci U S A. 96: 8150-5.
Zhou, H. et al. (2010) NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion.
J Pharmacol Exp Ther. 334: 260-8.
Kansanen, E. et al. (2009) Nrf2-dependent and -independent responses to nitro-fatty acids in human endothelial cells: identification of heat shock response as the major pathway activated by nitro-oleic acid.
J Biol Chem. 284: 33233-41.