Browsing Modes for Business Users

Notch 3 antibody | 487CT6.9.2

ISO 9001 Quality Assurance Manufacturing | Print
Anti Notch 3 (C-Terminal) Antibody, clone 487CT6.9.2 (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti Notch 3 (C-Terminal) Antibody, clone 487CT6.9.2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with notch 3 (C-terminal) antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 39 second exposure. Arrows points to notch 3 (C-terminal) (molecular weight 90, 270 kDa).

Mouse anti Notch 3 (C-Terminal)

Product Type
PrecisionAb Monoclonal
Clone
487CT6.9.2
Isotype
IgG1
Format
Purified
Specificity
Notch 3
Region
(C-TERMINAL)

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00484
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human notch 3 (C-Terminal) antibody recognizes notch 3, also known as neurogenic locus notch homolog protein 3.

The NOTCH3 gene encodes the third discovered human homologue of the Drosophila melanogaster type I membrane protein notch. In Drosophila, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in neural development. Homologues of the notch ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL1) (provided by RefSeq, Jul 2008).

Mouse anti Human notch 3 (C-Terminal) antibody detects bands of 90 and 270 kDa representing both cleaved and full length Notch 3. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti notch 3 (C-Terminal) detects bands of approximately 90 and 270 kDa in MCF7 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 2291-2321 of human notch 3
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q9UM47
Entrez Gene
NOTCH3
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0004872 receptor activity
GO:0005654 nucleoplasm
GO:0005788 endoplasmic reticulum lumen
GO:0005796 Golgi lumen
GO:0006350 transcription
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing

VMA00484

160712

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with NOTCH specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note