NOB1 antibody | F02/5F11

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Anti NOB1 Antibody, clone F02/5F11 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti NOB1 Antibody, clone F02/5F11 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NOB1 antibody (1/10000, **VMA00946**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/100000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to NOB1 (molecular weight 49 kDa)

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Mouse anti NOB1

Product Type: PrecisionAb Monoclonal
Clone: F02/5F11
Isotype: IgG1
Format: Purified
Specificity: NOB1

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VMA00946 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti NOB1 antibody recognizes RNA-binding protein NOB1, also known as ART4, NOB1P, and PSMD8BP1. NOB1 is a component of the 26S proteasome and plays an important role in protease function and RNA metabolism (Liu et al. 2015). It acts as a chaperone, joining the 20S proteasome with 19S regulatory particle in the nucleus and facilitating maturation of the 20S proteasome. NOB1 has been suggested to play a role in cell cycle progression, drug resistance and tumorigenesis (Li et al. 2013). Abnormal NOB1 expression may lead to dysfunctions in protein synthesis and protein degradation. Given that rapidly growing cancer cells have a high requirement for protein, dysregulation of NOB1 can be involved with cancer. Accordingly, many roles have been found for NOB1 in the development of cancer (Luo et al. 2018). NOB1 expression is upregulated in a variety of malignancies including prostate carcinoma, thyroid cancer, breast infiltrating ductal carcinoma and non-small lung cancer (Lin et al. 2015).

Target Species: Human
Western Blotting: Mouse anti NOB1 detects a band of approximately 49 kDa in HEK293 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-412 of human NOB1
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/10000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.