NOB1 antibody | F02/5F11

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Mouse anti NOB1

Product Type
PrecisionAb Monoclonal
Clone
F02/5F11
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00946
Datasheet
WB
SDS
100 µl loader

Mouse anti NOB1 antibody recognizes RNA-binding protein NOB1, also known as ART4, NOB1P, and PSMD8BP1. NOB1 is a component of the 26S proteasome and plays an important role in protease function and RNA metabolism (Liu et al. 2015). It acts as a chaperone, joining the 20S proteasome with 19S regulatory particle in the nucleus and facilitating maturation of the 20S proteasome. NOB1 has been suggested to play a role in cell cycle progression, drug resistance and tumorigenesis (Li et al. 2013). Abnormal NOB1 expression may lead to dysfunctions in protein synthesis and protein degradation. Given that rapidly growing cancer cells have a high requirement for protein, dysregulation of NOB1 can be involved with cancer. Accordingly, many roles have been found for NOB1 in the development of cancer (Luo et al. 2018). NOB1 expression is upregulated in a variety of malignancies including prostate carcinoma, thyroid cancer, breast infiltrating ductal carcinoma and non-small lung cancer (Lin et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti NOB1 detects a band of approximately 49 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-412 of human NOB1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9ULX3
Entrez Gene
NOB1
GO Terms
GO:0005515 protein binding
GO:0046872 metal ion binding
GO:0005634 nucleus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NOB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/10000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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