Nnmt antibody | EF02/2D5

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Mouse anti Nnmt

Product Type
PrecisionAb Monoclonal
Clone
EF02/2D5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00951
Datasheet
WB
SDS
100 µl loader

Mouse anti NNMT antibody recognizes nicotinamide N-methyltransferase (NNMT). NNMT is a cytosolic enzyme which catalyzes transfer of the methyl group from S-adenosyl-L-methionine (SAM) to nicotinamide (NA). SAM is a methyl donor for histones, non-histone proteins, DNA, RNA, lipids and other metabolites, and the activity of NNMT regulates gene expression via altered histone methylation (Eckert et al. 2019). NNMT has been found to promote migration, invasion, proliferation and survival of cancer cells. Accordingly, overexpression of NNMT has been documented in a variety of cancers of the lung, liver, kidney, bladder, and colon (Ulanovskaya et al. 2013). In addition to cancers, NNMT overexpression has been linked to several metabolic disorders. It has been suggested as a therapeutic target for treatment of cancers and metabolic disorders (Gao et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti NNMT detects a band of approximately 26 kDa in A549 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-264 of human NNMT
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P40261
Entrez Gene
NNMT
GO Terms
GO:0005829 cytosol
GO:0006805 xenobiotic metabolic process
GO:0008112 nicotinamide N-methyltransferase activity
GO:0030760 pyridine N-methyltransferase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Nnmt antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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