Nicastrin antibody | CD01/3H8
Mouse anti nicastrin antibody recognizes nicastrin, also known as NCT.
Nicastrin is the largest component of the gamma secetase complex, a complex that functions as a protease within the lipid bilayer to release secreted products. The gamma secretase complex is considered to be the "proteasome of the membrane" (Wolfe 2019). Nicastrin is essential for substrate recognition by gamma secretase, and has been termed the "gatekeeper" of the complex, physically limiting access to the catalytic activity of the protease and preventing proteins from being cut inappropriately (De Strooper, 2005; Urban, 2016). Appropriate proteolysis is relevant for Alzheimer's Disease, and continuous inhibition of nicastrin in the forebrain has been shown to lead no neurodegenerative features that resemble the neuron loss on Alzheimer's Disease (Ren et al. 2019). Nicastrin is overexpressed in breast cancer and relates to worse overall survival in a subset of patients. Inhibition of nicastrin has been suggested as a therapeutic goal in breast cancer (Lombardo et al. 2012).
Nicastrin is the largest component of the gamma secetase complex, a complex that functions as a protease within the lipid bilayer to release secreted products. The gamma secretase complex is considered to be the "proteasome of the membrane" (Wolfe 2019). Nicastrin is essential for substrate recognition by gamma secretase, and has been termed the "gatekeeper" of the complex, physically limiting access to the catalytic activity of the protease and preventing proteins from being cut inappropriately (De Strooper, 2005; Urban, 2016). Appropriate proteolysis is relevant for Alzheimer's Disease, and continuous inhibition of nicastrin in the forebrain has been shown to lead no neurodegenerative features that resemble the neuron loss on Alzheimer's Disease (Ren et al. 2019). Nicastrin is overexpressed in breast cancer and relates to worse overall survival in a subset of patients. Inhibition of nicastrin has been suggested as a therapeutic goal in breast cancer (Lombardo et al. 2012).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti nicastrin antibody detects a band of approximately 112 and 127 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 34-669 of human nicastrin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q92542
- Entrez Gene
- NCSTN
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005783 endoplasmic reticulum
- GO:0005887 integral to plasma membrane
- GO:0005794 Golgi apparatus
- GO:0006509 membrane protein ectodomain proteolysis
- GO:0007219 Notch signaling pathway
- GO:0007220 Notch receptor processing
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0016485 protein processing
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0042470 melanosome
- GO:0042987 amyloid precursor protein catabolic process
- GO:0043085 positive regulation of catalytic activity
- GO:0048011 nerve growth factor receptor signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Nicastrin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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