Nicastrin antibody | CD01/3H8

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Mouse anti Nicastrin

Product Type
PrecisionAb Monoclonal
Clone
CD01/3H8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00865
Datasheet
WB
SDS
100 µl loader

Mouse anti nicastrin antibody recognizes nicastrin, also known as NCT.

Nicastrin is the largest component of the gamma secetase complex, a complex that functions as a protease within the lipid bilayer to release secreted products. The gamma secretase complex is considered to be the "proteasome of the membrane" (Wolfe, 2019). Nicastrin is essential for substrate recognition by gamma secretase, and has been termed the "gatekeeper" of the complex, physically limiting access to the catalytic activity of the protease and preventing proteins from being cut inappropriately (De Strooper, 2005; Urban, 2016). Appropriate proteolysis is relevant for Alzheimer's Disease, and continuous inhibition of nicastrin in the forebrain has been shown to lead no neurodegenerative features that resemble the neuron loss on Alzheimer's Disease (Ren et al. 2019). Nicastrin is overexpressed in breast cancer and relates to worse overall survival in a subset of patients. Inhibition of nicastrin has been suggested as a therapeutic goal in breast cancer (Lombardo et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti nicastrin antibody detects a band of approximately 112 and 127 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 34-669 of human nicastrin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q92542
Entrez Gene
NCSTN
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005783 endoplasmic reticulum
GO:0005887 integral to plasma membrane
GO:0005794 Golgi apparatus
GO:0006509 membrane protein ectodomain proteolysis
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing
GO:0008624 induction of apoptosis by extracellular signals
GO:0016485 protein processing
GO:0031293 membrane protein intracellular domain proteolysis
GO:0042470 melanosome
GO:0042987 amyloid precursor protein catabolic process
GO:0043085 positive regulation of catalytic activity
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Nicastrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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