Nicastrin antibody | CD01/3H8

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Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with nicastrin antibody (1/1000, **VMA00865**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to nicastrin (molecular weight 112 and 127 kDa)

Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Nicastrin (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Nicastrin antibody (**VMA00865**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 98 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti Nicastrin Antibody, clone CD01/3H8 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of Nicastrin from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against NSCN (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti Nicastrin

Product Type: PrecisionAb Monoclonal
Clone: CD01/3H8
Isotype: IgG2b
Format: Purified
Specificity: Nicastrin

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00865 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti nicastrin antibody recognizes nicastrin, also known as NCT.

Nicastrin is the largest component of the gamma secetase complex, a complex that functions as a protease within the lipid bilayer to release secreted products. The gamma secretase complex is considered to be the "proteasome of the membrane" (Wolfe 2019). Nicastrin is essential for substrate recognition by gamma secretase, and has been termed the "gatekeeper" of the complex, physically limiting access to the catalytic activity of the protease and preventing proteins from being cut inappropriately (De Strooper, 2005; Urban, 2016). Appropriate proteolysis is relevant for Alzheimer's Disease, and continuous inhibition of nicastrin in the forebrain has been shown to lead no neurodegenerative features that resemble the neuron loss on Alzheimer's Disease (Ren et al. 2019). Nicastrin is overexpressed in breast cancer and relates to worse overall survival in a subset of patients. Inhibition of nicastrin has been suggested as a therapeutic goal in breast cancer (Lombardo et al. 2012).

Target Species: Human
Western Blotting: Mouse anti nicastrin antibody detects a band of approximately 112 and 127 kDa in HEK293 cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 34-669 of human nicastrin
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q92542
Entrez Gene: NCSTN
GO Terms: GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005783 endoplasmic reticulum; GO:0005887 integral to plasma membrane; GO:0005794 Golgi apparatus; GO:0006509 membrane protein ectodomain proteolysis; GO:0007219 Notch signaling pathway; GO:0007220 Notch receptor processing; GO:0008624 induction of apoptosis by extracellular signals; View More GO Terms; GO:0016485 protein processing; GO:0031293 membrane protein intracellular domain proteolysis; GO:0042470 melanosome; GO:0042987 amyloid precursor protein catabolic process; GO:0043085 positive regulation of catalytic activity; GO:0048011 nerve growth factor receptor signaling pathway