NHERF2 antibody

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Mouse anti NHERF2

Mouse anti human SLC9A3R2 antibody recognizes the Na(+)/H(+) exchange regulatory cofactor NHERF2, also known as NHE3 kinase A regulatory protein E3KARP, or tyrosine kinase activator protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

NHERF2 Antibody with Control Lysate

Mouse anti human SLC9A3R2 antibody recognizes the Na(+)/H(+) exchange regulatory cofactor NHERF2, also known as NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1 or tyrosine kinase activator protein 1. This antibody has been extensively validated for western blotting.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00097	IF WB	100 µl
VMA00097KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human NHERF2 recognizes NHERF2, also known as sodium-hydrogen exchange regulatory cofactor NHE-RF2. NHERF2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. In addition, it may also act as a scaffold protein in the nucleus.

NHERF2 is widely expressed in a number of tissues and within the cell it is localised to the endomembrane system, nucleus, apical cell membrane and the glomerular epithelium. Defects in the gene encoding NHERF2 are linked to a number of diseases including cystic fibrosis, hypertensive disease, fibrosis and acidosis amongst others.

Mouse anti Human NHERF2 antibody is specific for the second PDZ domain of NHERF2 and has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti NHERF2 antibody detects a band of approximately 46 kDa in Western Blotting
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation: 20μl mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant fusion protein corresponding to full length NHERF2, fused with maltose binding protein
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q15599 Related reagents
Entrez Gene: SLC9A3R2 Related reagents
GO Terms: GO:0005634 nucleus; GO:0006461 protein complex assembly; GO:0008022 protein C-terminus binding; GO:0012505 endomembrane system; GO:0016324 apical plasma membrane
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of NHERF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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