NHERF2 antibody

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Mouse anti NHERF2

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00097
Datasheet
IF WB
MSDS
100 µl loader

Mouse anti Human NHERF2 recognizes NHERF2, also known as sodium-hydrogen exchange regulatory cofactor NHE-RF2. NHERF2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. In addition, it may also act as a scaffold protein in the nucleus.

NHERF2 is widely expressed in a number of tissues and within the cell it is localised to the endomembrane system, nucleus, apical cell membrane and the glomerular epithelium. Defects in the gene encoding NHERF2 are linked to a number of diseases including cystic fibrosis, hypertensive disease, fibrosis and acidosis amongst others.

Mouse anti Human NHERF2 antibody is specific for the second PDZ domain of NHERF2 and has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti NHERF2 antibody detects a band of approximately 46 kDa in Western Blotting
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein corresponding to full length NHERF2, fused with maltose binding protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q15599 Related reagents
Entrez Gene
SLC9A3R2 Related reagents
GO Terms
GO:0005634 nucleus
GO:0006461 protein complex assembly
GO:0008022 protein C-terminus binding
GO:0012505 endomembrane system
GO:0016324 apical plasma membrane
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NHERF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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