NHERF2 antibody
Mouse anti NHERF2
Mouse anti human SLC9A3R2 antibody recognizes the Na(+)/H(+) exchange regulatory cofactor NHERF2, also known as NHE3 kinase A regulatory protein E3KARP, or tyrosine kinase activator protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00097 | IF WB | 100 µl |
Mouse anti Human NHERF2 recognizes NHERF2, also known as sodium-hydrogen exchange regulatory cofactor NHE-RF2. NHERF2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. In addition, it may also act as a scaffold protein in the nucleus.
NHERF2 is widely expressed in a number of tissues and within the cell it is localised to the endomembrane system, nucleus, apical cell membrane and the glomerular epithelium. Defects in the gene encoding NHERF2 are linked to a number of diseases including cystic fibrosis, hypertensive disease, fibrosis and acidosis amongst others.
Mouse anti Human NHERF2 antibody is specific for the second PDZ domain of NHERF2 and has been extensively validated for western blotting using whole cell lysates.
NHERF2 is widely expressed in a number of tissues and within the cell it is localised to the endomembrane system, nucleus, apical cell membrane and the glomerular epithelium. Defects in the gene encoding NHERF2 are linked to a number of diseases including cystic fibrosis, hypertensive disease, fibrosis and acidosis amongst others.
Mouse anti Human NHERF2 antibody is specific for the second PDZ domain of NHERF2 and has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NHERF2 antibody detects a band of approximately 46 kDa in Western Blotting
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fusion protein corresponding to full length NHERF2, fused with maltose binding protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q15599 Related reagents
- Entrez Gene
- SLC9A3R2 Related reagents
- GO Terms
- GO:0005634 nucleus
- GO:0006461 protein complex assembly
- GO:0008022 protein C-terminus binding
- GO:0012505 endomembrane system
- GO:0016324 apical plasma membrane
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of NHERF2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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