NGAL antibody | D11-6D10

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Mouse anti Human NGAL

Product Type
Monoclonal Antibody
Clone
D11-6D10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6198GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human NGAL antibody, clone D11-6D10, recognizes Neutrophil Gelatinase-Associated Lipocalin (NGAL) also known as lipocalin-2, 24 kDa alpha-2-microglobin-related subunit of MMP-9 and siderocalin. NGAL is a 198 amino acid 25 kDa secreted protein which possesses a lipid binding domain which enables it to bind small hydrophobic ligand molecules such as bacterial siderophores (Bauvois & Susin 2018, Abella et al. 2015). As well as the 25 kDa form, NGAL can exist as a 46 kDa homodimer and a 130 kDa heterodimer with MMP-9. The interaction with MMP-9 occurs in the extracellular space upon secretion of both proteins. It is through this interaction that MMP-9 proteolytic activity can be inhibited (Chakraborty et al. 2012).

NGAL is expressed in various cell types including adipocytes, hepatocytes, pneumocytes, splenocytes, mesangial and microglial cells. NGAL has also been observed circulating at low levels in urine and blood of healthy subjects (40-109 ng/ml) . However it is overexpressed during various inflammatory, metabolic, neurologic and cancer pathologies. The higher levels of NGAL expression in these diseases have been correlated with disease severity and poor prognosis (Bauvois & Susin 2018, Chakraborty et al. 2012).

The purified Mouse anti Human NGAL antibody, clone D11-6D10 (MCA6198GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human NGAL antibody, clone D02-7D4 (MCA6199B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human NGAL (Gln21-Gly198)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P80188
Entrez Gene
LCN2
Regulatory
For research purposes only

Applications of NGAL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human NGAL:Biotin MCA6199B E 0.1 mg loader
Recombinant Human NGAL PHP302 E 10 µg loader

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