NGAL antibody | D11-6D10

Mouse anti Human NGAL

Product Type
Monoclonal Antibody
Clone
D11-6D10
Isotype
IgG1
Specificity
NGAL

Product Code Applications Pack Size List Price Your Price Qty
MCA6198GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human NGAL antibody, clone D11-6D10, recognizes Neutrophil Gelatinase-Associated Lipocalin (NGAL) also known as lipocalin-2, 24 kDa alpha-2-microglobin-related subunit of MMP-9 and siderocalin. NGAL is a 198 amino acid 25 kDa secreted protein which possesses a lipid binding domain which enables it to bind small hydrophobic ligand molecules such as bacterial siderophores (Bauvois & Susin 2018, Abella et al. 2015). As well as the 25 kDa form, NGAL can exist as a 46 kDa homodimer and a 130 kDa heterodimer with MMP-9. The interaction with MMP-9 occurs in the extracellular space upon secretion of both proteins. It is through this interaction that MMP-9 proteolytic activity can be inhibited (Chakraborty et al. 2012).

NGAL is expressed in various cell types including adipocytes, hepatocytes, pneumocytes, splenocytes, mesangial and microglial cells. NGAL has also been observed circulating at low levels in urine and blood of healthy subjects (40-109 ng/ml) . However it is overexpressed during various inflammatory, metabolic, neurologic and cancer pathologies. The higher levels of NGAL expression in these diseases have been correlated with disease severity and poor prognosis (Bauvois & Susin 2018, Chakraborty et al. 2012).

The purified Mouse anti Human NGAL antibody, clone D11-6D10 (MCA6198GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human NGAL antibody, clone D02-7D4 (MCA6199B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human NGAL (Gln21-Gly198)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Detection Antibody: MCA6199B ELISA Antigen-Positive Control: PHP302
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human NGAL:Biotin MCA6199B E 0.1 mg loader
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Description Mouse anti Human NGAL:Biotin
Recombinant Human NGAL PHP302 E 10 µg loader
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Description Recombinant Human NGAL

UniProt
P80188
Entrez Gene
LCN2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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