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NFkB p52 antibody | 9D2

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Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of NFkB p52 CRISPR knockout HeLa (NFkB p52 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti NFkB p52 antibody (VMA00579) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 300 second and (B) 30 second exposure.
Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of NFkB p52 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti NFkB p52 Antibody, clone 9D2 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates probed with NFkB p52 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to NFkB p52 (molecular weight 52 kDa).

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WB

Mouse anti NFkB p52

Product Type
PrecisionAb Monoclonal
Clone
9D2
Isotype
IgG2b
Format
Purified
Specificity
NFkB p52

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Product Code Applications Pack Size List Price Your Price Qty
VMA00579
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human NFkB p52 antibody recognizes nuclear factor NF-kappa-B p100 subunit, also known as lymphocyte translocation chromosome 10 protein, nuclear factor Kappa-B subunit 2, nuclear factor of Kappa light chain gene enhancer in B cells 2, oncogene Lyt-10 or transcription factor NFKB2.

The NFKB2 gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB transcription factor complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both homo- or heterodimers which bind specific kappa-B elements in target genes. NFKB2 encodes the p100 subunit that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants (provided by RefSeq, May 2012).

Mouse anti Human NFkB p52 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti NFkB p52 detects a band of approximately 52 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
HEPES buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
E. coli-derived recombinant human NFkB p52 (amino acids 1 - 454)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q00653
Entrez Gene
NFKB2
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0033257 Bcl3/NF-kappaB2 complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0051092 positive regulation of NF-kappaB transcription factor activity

VMA00579

161202

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