NFE2L2 antibody

100% Secure


Mouse anti NFE2L2

NFE2L2 antibody recognizes nuclear factor erythroid 2-related factor 2, also known as HEBP1, NF-E2-related factor 2 or nuclear factor (erythroid-derived 2)-like 2. This antibody has been extensively validated for western blotting using whole cell lysates.

NFE2L2 Antibody with Control Lysate

NFE2L2 antibody recognizes nuclear factor erythroid 2-related factor 2, also known as HEBP1, NF-E2-related factor 2 or nuclear factor (erythroid-derived 2)-like 2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00224 WB 100 µl
VMA00224KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human NFE2L2 antibody recognizes the nuclear factor erythroid 2-related factor 2, also known as HEBP1, NF-E2-related factor 2, NFE2-related factor 2, nuclear factor (erythroid-derived 2)-like 2, nuclear factor erythroid-derived 2-like 2, nuclear factor and erythroid derived 2, like 2.

NFE2L2 gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for NFE2L2 (provided by RefSeq, Jan 2012).

Mouse anti Human NFE2L2 antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. The mouse anti Human NFE2L2 antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.

Product Details

Target Species
Human
Western Blotting
Anti NFE2L2 detects a band of approximately 68 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 356-589 of human NFE2L2 expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q16236 Related reagents
Entrez Gene
NFE2L2 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0005813 centrosome
GO:0006366 transcription from RNA polymerase II promoter
GO:0019904 protein domain specific binding
GO:0043565 sequence-specific DNA binding
GO:0046983 protein dimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NFE2L2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted