NF Kappa B Inhibitor Alpha antibody | 6A055

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Mouse anti NF Kappa B Inhibitor Alpha

Product Type
Monoclonal Antibody
Clone
6A055
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6019 IP WB 50 µg
Mouse anti NF kappa B inhibitor alpha antibody, clone 6A055, recognizes NF kappa B inhibitor alpha also known as I-kappa-B-alpha (IkB-alpha) and major histocompatibility complex enhancer-binding protein MAD3. NF kappa B inhibitor alpha is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NF kappa B inhibitor alpha inhibits the NF kappa B complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NF kappa B inhibitor alpha is phosphorylated so that it dissociates from NF kappa B and is then ubiquinated prior to degradation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Thiomersal
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant human NF kappa B inhibitor alpha
Approx. Protein Concentrations
IgG concentration 0.25 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P25963 Related reagents
Entrez Gene
NFKBIA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0008063 Toll signaling pathway
GO:0008139 nuclear localization sequence binding
GO:0042802 identical protein binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010875 positive regulation of cholesterol efflux
GO:0010888 negative regulation of lipid storage
GO:0031625 ubiquitin protein ligase binding
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032270 positive regulation of cellular protein metabolic process
GO:0033256 I-kappaB/NF-kappaB complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory
For research purposes only

Applications of NF Kappa B Inhibitor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 1.0 4.0 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse anti Human NF Kappa B Inhibitor alpha antibody recognizes both the phosphorylated and non-phosphorylated forms of NF Kappa B Inhibitor alpha (approximately 35 kDa) in untreated Jurkat cell lysate and lysate from Jurkat cells treated with TNFα.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F