Neuron Specific Enolase antibody

Mouse anti Neuron Specific Enolase

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Specificity
Neuron Specific Enolase

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VMA00069
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WB 100 µl loader
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Mouse anti Human neuron specific enolase antibody recognizes neuron specific enolase (NSE), also known as gamma-enolase, 2-phospho-D-glycerate hydro-lyase, enolase 2 and neural enolase.

Encoded by the ENO2 gene, NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates (provided by RefSeq, Jul 2008).

Mouse anti Human neuron specific enolase antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti neuron specific enolase detects a band of approximately 47 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Native protein from brain
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P09104
Entrez Gene
ENO2
GO Terms
GO:0000015 phosphopyruvate hydratase complex
GO:0005886 plasma membrane
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0006094 gluconeogenesis
GO:0006096 glycolysis