Neurofilament antibody | OTI3G2

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Mouse anti Neurofilament

Neurofilament antibody recognizes neurofilament light polypeptide, also known as light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, or regulatory subunit 110.

Product Type
PrecisionAb™ Monoclonal
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00189 WB 100 µl
Mouse anti Human neurofilament antibody recognizes neurofilament, also known as light molecular weight neurofilament protein, neurofilament light polypeptide, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptide 68kDa and protein phosphatase 1 regulatory subunit 110.

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. The NEFL gene encodes the light chain neurofilament protein. Mutations in NEFL cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y (provided by RefSeq, Oct 2008).

Mouse anti Human neurofilament antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Anti neurofilament detects a band of approximately 70 kDa in mouse brain cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Recombinant protein fragment corresponding to amino acids 1-360 of human neurofilament (NP_006149) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

P07196 Related reagents
Entrez Gene
NEFL Related reagents
GO Terms
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005883 neurofilament
GO:0007268 synaptic transmission
GO:0008022 protein C-terminus binding
GO:0008089 anterograde axon cargo transport
GO:0008090 retrograde axon cargo transport
GO:0042802 identical protein binding
GO:0019896 axon transport of mitochondrion
GO:0033693 neurofilament bundle assembly
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of Neurofilament antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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