Neurofilament antibody | OTI3G2
Mouse anti Neurofilament
Neurofilament antibody recognizes neurofilament light polypeptide, also known as light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, or regulatory subunit 110.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3G2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00189 | WB | 100 µl |
Mouse anti Human neurofilament antibody recognizes neurofilament, also known as light molecular weight neurofilament protein, neurofilament light polypeptide, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptide 68kDa and protein phosphatase 1 regulatory subunit 110.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. The NEFL gene encodes the light chain neurofilament protein. Mutations in NEFL cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y (provided by RefSeq, Oct 2008).
Mouse anti Human neurofilament antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. The NEFL gene encodes the light chain neurofilament protein. Mutations in NEFL cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y (provided by RefSeq, Oct 2008).
Mouse anti Human neurofilament antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti neurofilament detects a band of approximately 70 kDa in mouse brain cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to amino acids 1-360 of human neurofilament (NP_006149) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P07196 Related reagents
- Entrez Gene
- NEFL Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005883 neurofilament
- GO:0007268 synaptic transmission
- GO:0008022 protein C-terminus binding
- GO:0008089 anterograde axon cargo transport
- GO:0008090 retrograde axon cargo transport
- GO:0042802 identical protein binding
- GO:0019896 axon transport of mitochondrion
- GO:0033693 neurofilament bundle assembly
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Neurofilament antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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