Neurofilament antibody | OTI3G2

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Anti Neurofilament Antibody, clone OTI3G2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Neurofilament Antibody, clone OTI3G2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human neurofilament antibody, clone OTI3G2 (**VMA00189**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to neurofilament (molecular weight 70 kDa).

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Mouse anti Neurofilament

Product Type: PrecisionAb Monoclonal
Clone: OTI3G2
Isotype: IgG1
Format: Purified
Specificity: Neurofilament

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VMA00189 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human neurofilament antibody, clone OTI3G2 recognizes neurofilament, also known as light molecular weight neurofilament protein, neurofilament light polypeptide, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptide 68kDa and protein phosphatase 1 regulatory subunit 110.

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. The NEFL gene encodes the light chain neurofilament protein. Mutations in NEFL cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y (provided by RefSeq, Oct 2008).

Mouse anti Human neurofilament antibody, clone OTI3G2 detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti neurofilament detects a band of approximately 70 kDa in mouse brain cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to amino acids 1-360 of human neurofilament (NP_006149) produced in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P07196
Entrez Gene: NEFL
GO Terms: GO:0005829 cytosol; GO:0005200 structural constituent of cytoskeleton; GO:0005883 neurofilament; GO:0007268 synaptic transmission; GO:0008022 protein C-terminus binding; GO:0008089 anterograde axon cargo transport; GO:0008090 retrograde axon cargo transport; GO:0042802 identical protein binding; GO:0019896 axon transport of mitochondrion; View More GO Terms; GO:0033693 neurofilament bundle assembly