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Nemo-Like Kinase antibody

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Western Blot Validated
Anti Nemo-Like Kinase Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with nemo-like kinase antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to nemo-like kinase (molecular weight 68 kDa)

Mouse anti Nemo-Like Kinase

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00357
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human nemo-like kinase antibody recognizes the serine/threonine-protein kinase nemo-like kinase.

Mouse anti Human nemo-like kinase antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti nemo-like kinase detects a band of approximately 68 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified His-tagged nemo-like kinase protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UBE8 Related reagents
Entrez Gene
NLK Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0004707 MAP kinase activity
GO:0016055 Wnt receptor signaling pathway
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0008134 transcription factor binding
GO:0018107 peptidyl-threonine phosphorylation
GO:0030178 negative regulation of Wnt receptor signaling pathway
GO:0031625 ubiquitin protein ligase binding
GO:0033136 serine phosphorylation of STAT3 protein
GO:0042169 SH2 domain binding
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Nemo-Like Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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