Nemo-Like Kinase antibody
Mouse anti Nemo-Like Kinase
Mouse anti human nemo-like kinase antibody recognizes the serine/threonine-protein kinase NLK. This antibody has been extensively validated for western blotting using whole cell lysates.
Nemo-Like Kinase Antibody with Control Lysate
Mouse anti human nemo-like kinase antibody recognizes the serine/threonine-protein kinase NLK. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG2a
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00357 | WB | 100 µl | ||||
| VMA00357KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human nemo-like kinase antibody recognizes the serine/threonine-protein kinase nemo-like kinase.
Mouse anti Human nemo-like kinase antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human nemo-like kinase antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti nemo-like kinase detects a band of approximately 68 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified His-tagged nemo-like kinase protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9UBE8 Related reagents
- Entrez Gene
- NLK Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004707 MAP kinase activity
- GO:0016055 Wnt receptor signaling pathway
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006350 transcription
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0008134 transcription factor binding
- GO:0018107 peptidyl-threonine phosphorylation
- GO:0030178 negative regulation of Wnt receptor signaling pathway
- GO:0031625 ubiquitin protein ligase binding
- GO:0033136 serine phosphorylation of STAT3 protein
- GO:0042169 SH2 domain binding
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Nemo-Like Kinase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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