The protein encoded by NCK1 gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found (provided by RefSeq, Jun 2010).
Mouse anti Human NCK antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti NCK detects a band of approximately 47 kDa in Jurkat cell lysates
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- E. coli-derived recombinant human NCK
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P16333 Related reagents
- Entrez Gene
- NCK1 Related reagents
- GO Terms
- GO:0005102 receptor binding
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005634 nucleus
- GO:0006417 regulation of translation
- GO:0007172 signal complex assembly
- GO:0007411 axon guidance
- GO:0008093 cytoskeletal adaptor activity
- GO:0030159 receptor signaling complex scaffold activity
- GO:0030838 positive regulation of actin filament polymerization
- GO:0042102 positive regulation of T cell proliferation
- GO:0042110 T cell activation
- GO:0050852 T cell receptor signaling pathway
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of Nck antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||2 ml||WB|
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