NBS1 antibody | UMAB100

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Anti NBS1 Antibody, clone UMAB100 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NBS1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to NBS1 (molecular weight 95 kDa)

Mouse anti NBS1

Mouse anti human NBS1 antibody recognizes the protein nibrin, also known as NBN, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 or p95 protein of the MRE11/RAD50 complex. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: UMAB100
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00403 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human NBS1 antibody recognizes NBS1, also known as nibrin, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 and p95 protein of the MRE11/RAD50 complex.

Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).

Mouse anti Human NBS1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti NBS1 detects a band of approximately 95 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to aa 183-460 of human NBS1 (NP_002476) produced in E.coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: O60934 Related reagents
Entrez Gene: NBN Related reagents
GO Terms: GO:0000723 telomere maintenance; GO:0000724 double-strand break repair via homologous recombination; GO:0000784 nuclear chromosome, telomeric region; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0007050 cell cycle arrest; GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint; GO:0007126 meiosis; GO:0008134 transcription factor binding; GO:0030174 regulation of DNA-dependent DNA replication initiation; GO:0030330 DNA damage response, signal transduction by p53 class mediator; GO:0030870 Mre11 complex; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031954 positive regulation of protein autophosphorylation; GO:0032508 DNA duplex unwinding; GO:0033674 positive regulation of kinase activity; GO:0042405 nuclear inclusion body; GO:0047485 protein N-terminus binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of NBS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in		Get Quote

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