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NBS1 antibody | UMAB100

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Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with NBS1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to NBS1 (molecular weight 95 kDa).
Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of NBS1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti NBS1 antibody (VMA00403) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 79 second and (B) 30 second exposure.
Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of NBS1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti NBS1

Product Type
PrecisionAb Monoclonal
Clone
UMAB100
Isotype
IgG1
Format
Purified
Specificity
NBS1

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00403
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human NBS1 antibody, clone UMAB100 recognizes NBS1, also known as nibrin, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 and p95 protein of the MRE11/RAD50 complex.

Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).

Mouse anti Human NBS1 antibody, clone UMAB100 detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti NBS1 detects a band of approximately 95 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 183-460 of human NBS1 (NP_002476) produced in E.coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
O60934
Entrez Gene
NBN
GO Terms
GO:0000723 telomere maintenance
GO:0000724 double-strand break repair via homologous recombination
GO:0000784 nuclear chromosome, telomeric region
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0007050 cell cycle arrest
GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
GO:0007126 meiosis
GO:0008134 transcription factor binding
GO:0030174 regulation of DNA-dependent DNA replication initiation
GO:0030330 DNA damage response, signal transduction by p53 class mediator
GO:0030870 Mre11 complex
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0033674 positive regulation of kinase activity
GO:0042405 nuclear inclusion body
GO:0047485 protein N-terminus binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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