NBS1 antibody | UMAB100
Mouse anti NBS1
Mouse anti human NBS1 antibody recognizes the protein nibrin, also known as NBN, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 or p95 protein of the MRE11/RAD50 complex. This antibody has been extensively validated for western blotting using whole cell lysates.
NBS1 Antibody with Control Lysate
Mouse anti human NBS1 antibody recognizes the protein nibrin, also known as NBN, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 or p95 protein of the MRE11/RAD50 complex. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- UMAB100
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00403 | WB | 100 µl | ||||
| VMA00403KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human NBS1 antibody recognizes NBS1, also known as nibrin, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 and p95 protein of the MRE11/RAD50 complex.
Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).
Mouse anti Human NBS1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).
Mouse anti Human NBS1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NBS1 detects a band of approximately 95 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein fragment corresponding to aa 183-460 of human NBS1 (NP_002476) produced in E.coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O60934 Related reagents
- Entrez Gene
- NBN Related reagents
- GO Terms
- GO:0000723 telomere maintenance
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000784 nuclear chromosome, telomeric region
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0007050 cell cycle arrest
- GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
- GO:0007126 meiosis
- GO:0008134 transcription factor binding
- GO:0030174 regulation of DNA-dependent DNA replication initiation
- GO:0030330 DNA damage response, signal transduction by p53 class mediator
- GO:0030870 Mre11 complex
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0031954 positive regulation of protein autophosphorylation
- GO:0032508 DNA duplex unwinding
- GO:0033674 positive regulation of kinase activity
- GO:0042405 nuclear inclusion body
- GO:0047485 protein N-terminus binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of NBS1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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