NBS1 antibody | UMAB100

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Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with NBS1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to NBS1 (molecular weight 95 kDa).

Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of NBS1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti NBS1 antibody (**VMA00403**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 79 second and (B) 30 second exposure.

Anti NBS1 Antibody, clone UMAB100 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of NBS1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Mouse anti NBS1

Product Type: PrecisionAb Monoclonal
Clone: UMAB100
Isotype: IgG1
Format: Purified
Specificity: NBS1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00403 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human NBS1 antibody, clone UMAB100 recognizes NBS1, also known as nibrin, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 and p95 protein of the MRE11/RAD50 complex.

Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).

Mouse anti Human NBS1 antibody, clone UMAB100 detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti NBS1 detects a band of approximately 95 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to aa 183-460 of human NBS1 (NP_002476) produced in E.coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: O60934
Entrez Gene: NBN
GO Terms: GO:0000723 telomere maintenance; GO:0000724 double-strand break repair via homologous recombination; GO:0000784 nuclear chromosome, telomeric region; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0007050 cell cycle arrest; GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint; GO:0007126 meiosis; GO:0008134 transcription factor binding; View More GO Terms; GO:0030174 regulation of DNA-dependent DNA replication initiation; GO:0030330 DNA damage response, signal transduction by p53 class mediator; GO:0030870 Mre11 complex; GO:0031575 mitotic cell cycle G1/S transition checkpoint; GO:0031954 positive regulation of protein autophosphorylation; GO:0032508 DNA duplex unwinding; GO:0033674 positive regulation of kinase activity; GO:0042405 nuclear inclusion body; GO:0047485 protein N-terminus binding