NBS1 antibody | UMAB100

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Mouse anti NBS1

Mouse anti human NBS1 antibody recognizes the protein nibrin, also known as NBN, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 or p95 protein of the MRE11/RAD50 complex. This antibody has been extensively validated for western blotting using whole cell lysates.

NBS1 Antibody with Control Lysate

Mouse anti human NBS1 antibody recognizes the protein nibrin, also known as NBN, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 or p95 protein of the MRE11/RAD50 complex. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
UMAB100
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00403 WB 100 µl
VMA00403KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human NBS1 antibody recognizes NBS1, also known as nibrin, Nijmegen breakage syndrome 1, cell cycle regulatory protein p95 and p95 protein of the MRE11/RAD50 complex.

Mutations in the NBN gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. The NBN gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008).

Mouse anti Human NBS1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti NBS1 detects a band of approximately 95 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 183-460 of human NBS1 (NP_002476) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O60934 Related reagents
Entrez Gene
NBN Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000724 double-strand break repair via homologous recombination
GO:0000784 nuclear chromosome, telomeric region
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0007050 cell cycle arrest
GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
GO:0007126 meiosis
GO:0008134 transcription factor binding
GO:0030174 regulation of DNA-dependent DNA replication initiation
GO:0030330 DNA damage response, signal transduction by p53 class mediator
GO:0030870 Mre11 complex
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0033674 positive regulation of kinase activity
GO:0042405 nuclear inclusion body
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of NBS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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