NBS1 antibody | AB02/3A3

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Mouse anti NBS1

Product Type
PrecisionAb™ Monoclonal
Clone
AB02/3A3
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00524
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human NBS1 antibody recognizes nibrin, also known as ATV, NBN or p95.

Mutations in the NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. NBS1 is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation (provided by RefSeq, Jul 2008)

Mouse anti Human NBS1 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti NBS1 detects a band of approximately 95 kDa in K562 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human NBS1 (amino acids 461 - 754)

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O60934 Related reagents
Entrez Gene
NBN Related reagents
GO Terms
GO:0000723 telomere maintenance
GO:0000724 double-strand break repair via homologous recombination
GO:0000784 nuclear chromosome, telomeric region
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0007050 cell cycle arrest
GO:0007095 mitotic cell cycle G2/M transition DNA damage checkpoint
GO:0007126 meiosis
GO:0008134 transcription factor binding
GO:0030174 regulation of DNA-dependent DNA replication initiation
GO:0030330 DNA damage response, signal transduction by p53 class mediator
GO:0030870 Mre11 complex
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0031954 positive regulation of protein autophosphorylation
GO:0032508 DNA duplex unwinding
GO:0033674 positive regulation of kinase activity
GO:0042405 nuclear inclusion body
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of NBS1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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