N-Cadherin antibody | OTI2G7

Mouse anti N-Cadherin

Product Type
PrecisionAb Monoclonal
Clone
OTI2G7
Isotype
IgG2a
Format
Purified
Specificity
N-Cadherin

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VMA00601
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human N-cadherin antibody recognizes N-cadherin, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal and neural cadherin.

N-cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by CDH2 product (provided by RefSeq, Jul 2008).

Mouse anti Human N-cadherin antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti N-cadherin antibody detects a band of approximately 140 kDa in C6 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived full length recombinant human N-cadherin
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19022
Entrez Gene
CDH2
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0008013 beta-catenin binding
GO:0016342 catenin complex
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042692 muscle cell differentiation
GO:0045294 alpha-catenin binding
GO:0045295 gamma-catenin binding
GO:0051149 positive regulation of muscle cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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