N-Cadherin antibody | OTI2G7

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Mouse anti N-Cadherin

N-Cadherin antibody recognizes N-cadherin-2, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal or neural cadherin.

N-Cadherin Antibody with Control Lysate

N-Cadherin antibody recognizes N-cadherin-2, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal or neural cadherin.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2G7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00601 WB 100 µl
VMA00601KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human N-cadherin antibody recognizes N-cadherin, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal and neural cadherin.

N-cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by CDH2 product (provided by RefSeq, Jul 2008).

Mouse anti Human N-cadherin antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
HEK293-derived full length recombinant human N-cadherin

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P19022 Related reagents
Entrez Gene
CDH2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0008013 beta-catenin binding
GO:0016342 catenin complex
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042692 muscle cell differentiation
GO:0045294 alpha-catenin binding
GO:0045295 gamma-catenin binding
GO:0051149 positive regulation of muscle cell differentiation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of N-Cadherin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti N-cadherin antibody detects a band of approximately 140 kDa in C6 cell lysates
Western Blotting
Mouse anti N-cadherin antibody detects a band of approximately 140 kDa in C6 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB