Myocyte-Specific Enhancer Factor 2D antibody | OTI3D12

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Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human myocyte-specific enhancer factor 2D antibody, clone OTI3D12 (**VMA00268**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to myocyte-specific enhancer factor 2D (molecular weight 70 kDa).

Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Myocyte-Specific Enhancer Factor 2D (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Myocyte-Specific Enhancer Factor 2D antibody (**VMA00268**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 120 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.

Anti Myocyte-Specific Enhancer Factor 2D Antibody, clone OTI3D12 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of Myocyte-Specific Enhancer Factor 2D from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Myocyte-Specific Enhancer Factor 2D (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti Myocyte-Specific Enhancer Factor 2D

Product Type: PrecisionAb Monoclonal
Clone: OTI3D12
Isotype: IgG1
Format: Purified
Specificity: Myocyte-Specific Enhancer Factor 2D

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VMA00268 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human myocyte-specific enhancer factor 2D antibody, clone OTI3D12 recognizes myocyte-specific enhancer factor 2D, also known as MADS box transcription enhancer factor 2, polypeptide D and myocyte-specific enhancer factor 2D.

MEF2D encodes a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson's disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants (provided by RefSeq, Oct 2012).

Mouse anti Human myocyte-specific enhancer factor 2D antibody, clone OTI3D12 detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti myocyte-specific enhancer factor 2D detects a band of approximately 70 kDa in RD cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-282 of human myocyte-specific enhancer factor 2D (NP_005911) produced in E. coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14814
Entrez Gene: MEF2D
GO Terms: GO:0005515 protein binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006915 apoptosis; GO:0005634 nucleus; GO:0007399 nervous system development; GO:0007517 muscle organ development; GO:0043565 sequence-specific DNA binding