Myocyte-Specific Enhancer Factor 2D antibody | OTI3D12

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Mouse anti Myocyte-Specific Enhancer Factor 2D

Myocyte-specific enhancer factor 2D antibody recognizes myocyte-specific enhancer factor 2D, also known as MADS box transcription enhancer factor 2 or polypeptide D. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3D12
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00268 WB 100 µl
Mouse anti Human myocyte-specific enhancer factor 2D antibody recognizes myocyte-specific enhancer factor 2D, also known as MADS box transcription enhancer factor 2, polypeptide D and myocyte-specific enhancer factor 2D.

MEF2D encodes a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson's disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants (provided by RefSeq, Oct 2012).

Mouse anti Human myocyte-specific enhancer factor 2D antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti myocyte-specific enhancer factor 2D detects a band of approximately 70 kDa in RD cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-282 of human myocyte-specific enhancer factor 2D (NP_005911) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14814 Related reagents
Entrez Gene
MEF2D Related reagents
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006915 apoptosis
GO:0005634 nucleus
GO:0007399 nervous system development
GO:0007517 muscle organ development
GO:0043565 sequence-specific DNA binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Myocyte-Specific Enhancer Factor 2D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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