MyD88 antibody | KL01/3A2
Mouse anti MyD88 antibody recognizes myeloid differentiation primary response protein MyD88. MyD88 is the primary mediator of inflammatory pathways downstream of the Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor families, achieving these functions by activating IRAK family kinases. Accordingly, MyD88 plays an important role in innate immune signaling (Deguine and Barton 2014). MyD88 is also able to modulate energy, glucose, and lipid metabolism, acting as a nutrient sensor in the intestinal epithelium and controlling energy expenditure (Lefort et al. 2019). A role in immune response to allergens has also been identified for MyD88 (Thomas et al. 2018). Disruption of the gene encoding MyD88 has been found to suppress a range of tumor types in mice. Accordingly, it has been suggested that signaling through MyD88 promotes tumorigenesis (Echizen et al. 2016). Consistent with this, mutations in the gene encoding MyD88 leads to cancer development (Hindi et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti MyD88 detects a band of approximately 36 kDa in Jurkat cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-296 of human MyD88
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99836
- Entrez Gene
- MYD88
- GO Terms
- GO:0005886 plasma membrane
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004888 transmembrane receptor activity
- GO:0005123 death receptor binding
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0010008 endosome membrane
- GO:0031224 intrinsic to membrane
- GO:0032740 positive regulation of interleukin-17 production
- GO:0032747 positive regulation of interleukin-23 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050727 regulation of inflammatory response
- GO:0070555 response to interleukin-1
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of MyD88 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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