Mucin 4 antibody | 5B12
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P WB ResetMouse anti Human Mucin 4:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 5B12
- Isotype
- IgG2a
- Specificity
- Mucin 4
Antibody Quality and 
Unrivaled 
| Mouse anti Human Mucin 4 antibody, clone 5B12 recognizes human Mucin-4, also known as Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Testis mucin or Tracheobronchial mucin. Mucin 4 is a 2141 amino acid ~130 kDa single pass, type I transmembrane glycoprotein involved in the repression of apoptosis and in cell proliferation (Carraway et al. 2002). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 79-189 of human MUC-4
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Kim, S.M. et al. (2012) Expression of MUC1 and MUC4 in gallbladder adenocarcinoma.
Korean J Pathol. 46 (5): 429-35.
- Synonyms
- MUC-4
- RRID
- AB_2146692
- UniProt
- Q99102
- Entrez Gene
- MUC4
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