MTDH antibody | OTI1E7

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Anti MTDH Antibody, clone OTI1E7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MTDH Antibody, clone OTI1E7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MTDH Antibody, clone OTI1E7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human MTDH antibody, clone OTI1E7 (**VMA00204**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to MTDH (molecular weight 75 kDa).

Anti MTDH Antibody, clone OTI1E7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of MTDH from HEK293 whole cell lysate. The Input lane is 10% input; the MTDH-IP lane is the immunoprecipitation with the MTDH antibody (2.5 µg, **VMA00204**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using MTDH antibody (1/2,000, **VMA00204**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MTDH.

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WB IP

Mouse anti MTDH

Product Type: PrecisionAb Monoclonal
Clone: OTI1E7
Isotype: IgG1
Format: Purified
Specificity: MTDH

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00204 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human MTDH antibody, clone OTI1E7 recognizes MTDH, also known as 3D3/LYRIC, astrocyte elevated gene 1, astrocyte elevated gene-1 protein, lysine-rich CEACAM1 co-isolated protein, metastasis adhesion protein and protein LYRIC.

Mouse anti Human MTDH antibody, clone OTI1E7 detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MTDH detects a band of approximately 75 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 70-582 of human MTDH (NP_848927) produced in SF9 cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
List Price	Your Price
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q86UE4
Entrez Gene: MTDH
GO Terms: GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0001085 RNA polymerase II transcription factor binding; GO:0003713 transcription coactivator activity; GO:0016021 integral to membrane; GO:0005730 nucleolus; GO:0005789 endoplasmic reticulum membrane; GO:0005923 tight junction; GO:0010508 positive regulation of autophagy; GO:0016324 apical plasma membrane; View More GO Terms; GO:0016604 nuclear body; GO:0031663 lipopolysaccharide-mediated signaling pathway; GO:0031965 nuclear membrane; GO:0043066 negative regulation of apoptosis; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045766 positive regulation of angiogenesis; GO:0046581 intercellular canaliculus; GO:0048471 perinuclear region of cytoplasm; GO:0051059 NF-kappaB binding; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051897 positive regulation of protein kinase B signaling cascade