MSH6 antibody

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Mouse anti MSH6

MSH6 antibody recognizes the DNA mismatch repair protein MSH6, also known as G/T mismatch-binding protein or mutS-alpha 160 kDa subunit. This antibody has been extensively validated for western blotting using whole cell lysates.

MSH6 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00250	WB	100 µl
VMA00250KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human MSH6 antibody recognizes the DNA mismatch repair protein MSH6, also known as DNA mismatch repair protein MSH6, G/T mismatch-binding protein, mutS-alpha 160 kDa subunit, sperm-associated protein.

The MSH6 gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in MSH6 may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. (provided by RefSeq, Jul 2013).

Mouse anti Human MSH6 antibody detects a band of 160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti MSH6 detects a band of approximately 160 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation: 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified recombinant fragment of human MSH6 expressed in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P52701 Related reagents
Entrez Gene: MSH6 Related reagents
GO Terms: GO:0005515 protein binding; GO:0000710 meiotic mismatch repair; GO:0005524 ATP binding; GO:0007131 reciprocal meiotic recombination; GO:0008094 DNA-dependent ATPase activity; GO:0008340 determination of adult lifespan; GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis; GO:0009411 response to UV; GO:0016446 somatic hypermutation of immunoglobulin genes; GO:0032301 MutSalpha complex; GO:0045190 isotype switching; GO:0045910 negative regulation of DNA recombination; GO:0051096 positive regulation of helicase activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of MSH6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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