MSH2 antibody | 1B3A8A8
KO Validated
Mouse anti Human MSH2 antibody recognizes the DNA mismatch repair protein Msh2, also known as DNA mismatch repair protein Msh2, hMSH2, mutS homolog 2 and colon cancer, nonpolyposis type 1.
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for MSH2 (provided by RefSeq, Apr 2012).
Mouse anti Human MSH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for MSH2 (provided by RefSeq, Apr 2012).
Mouse anti Human MSH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti MSH2 detects a band of approximately 105 kDa in HeLa cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment of human MSH2 expressed in E. coli
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43246
- Entrez Gene
- MSH2
- GO Terms
- GO:0000228 nuclear chromosome
- GO:0000403 Y-form DNA binding
- GO:0000404 loop DNA binding
- GO:0000406 double-strand/single-strand DNA junction binding
- GO:0000710 meiotic mismatch repair
- GO:0005524 ATP binding
- GO:0006301 postreplication repair
- GO:0006302 double-strand break repair
- GO:0006311 meiotic gene conversion
- GO:0008022 protein C-terminus binding
- GO:0008094 DNA-dependent ATPase activity
- GO:0008584 male gonad development
- GO:0010165 response to X-ray
- GO:0010224 response to UV-B
- GO:0016446 somatic hypermutation of immunoglobulin genes
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0031573 intra-S DNA damage checkpoint
- GO:0032137 guanine/thymine mispair binding
- GO:0032301 MutSalpha complex
- GO:0032302 MutSbeta complex
- GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
- GO:0042803 protein homodimerization activity
- GO:0043524 negative regulation of neuron apoptosis
- GO:0043570 maintenance of DNA repeat elements
- GO:0045128 negative regulation of reciprocal meiotic recombination
- GO:0045190 isotype switching
- GO:0051096 positive regulation of helicase activity
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of MSH2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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