MSH2 antibody | 1B3A8A8

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Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to MSH2 (molecular weight 105 kDa)

Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of control and MSH2 knockout eHAP whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MSH2 (molecular weight 105 kDa).

Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 3 — Immunoprecipitation of MSH2 from HeLa whole cell lysate. The Input lane is 10% input; the MSH2-IP lane is the immunoprecipitation with the MSH2 antibody (2.5 µg, **VMA00286**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using MSH2 antibody (1/1,000, **VMA00286**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MSH2.

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Mouse anti MSH2

Product Type: PrecisionAb Monoclonal
Clone: 1B3A8A8
Isotype: IgG1
Format: Purified
Specificity: MSH2

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00286 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human MSH2 antibody recognizes the DNA mismatch repair protein Msh2, also known as DNA mismatch repair protein Msh2, hMSH2, mutS homolog 2 and colon cancer, nonpolyposis type 1.

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for MSH2 (provided by RefSeq, Apr 2012).

Mouse anti Human MSH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MSH2 detects a band of approximately 105 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant fragment of human MSH2 expressed in E. coli
Approx. Protein Concentrations: 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P43246
Entrez Gene: MSH2
GO Terms: GO:0000228 nuclear chromosome; GO:0000403 Y-form DNA binding; GO:0000404 loop DNA binding; GO:0000406 double-strand/single-strand DNA junction binding; GO:0000710 meiotic mismatch repair; GO:0005524 ATP binding; GO:0006301 postreplication repair; GO:0006302 double-strand break repair; GO:0006311 meiotic gene conversion; View More GO Terms; GO:0008022 protein C-terminus binding; GO:0008094 DNA-dependent ATPase activity; GO:0008584 male gonad development; GO:0010165 response to X-ray; GO:0010224 response to UV-B; GO:0016446 somatic hypermutation of immunoglobulin genes; GO:0019901 protein kinase binding; GO:0030183 B cell differentiation; GO:0031573 intra-S DNA damage checkpoint; GO:0032137 guanine/thymine mispair binding; GO:0032301 MutSalpha complex; GO:0032302 MutSbeta complex; GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; GO:0042803 protein homodimerization activity; GO:0043524 negative regulation of neuron apoptosis; GO:0043570 maintenance of DNA repeat elements; GO:0045128 negative regulation of reciprocal meiotic recombination; GO:0045190 isotype switching; GO:0051096 positive regulation of helicase activity