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MSH2 antibody | 1B3A8A8

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Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to MSH2 (molecular weight 105 kDa)
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of control and MSH2 knockout eHAP whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MSH2 (molecular weight 105 kDa).
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 3
Immunoprecipitation of MSH2 from HeLa whole cell lysate. The Input lane is 10% input; the MSH2-IP lane is the immunoprecipitation with the MSH2 antibody (2.5 µg, VMA00286); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using MSH2 antibody (1/1,000, VMA00286) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MSH2.

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WB IP

Mouse anti MSH2

Product Type
PrecisionAb Monoclonal
Clone
1B3A8A8
Isotype
IgG1
Format
Purified
Specificity
MSH2

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00286
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader
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Mouse anti Human MSH2 antibody recognizes the DNA mismatch repair protein Msh2, also known as DNA mismatch repair protein Msh2, hMSH2, mutS homolog 2 and colon cancer, nonpolyposis type 1.

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for MSH2 (provided by RefSeq, Apr 2012).

Mouse anti Human MSH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MSH2 detects a band of approximately 105 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human MSH2 expressed in E. coli
Approx. Protein Concentrations
0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P43246
Entrez Gene
MSH2
GO Terms
GO:0000228 nuclear chromosome
GO:0000403 Y-form DNA binding
GO:0000404 loop DNA binding
GO:0000406 double-strand/single-strand DNA junction binding
GO:0000710 meiotic mismatch repair
GO:0005524 ATP binding
GO:0006301 postreplication repair
GO:0006302 double-strand break repair
GO:0006311 meiotic gene conversion
GO:0008022 protein C-terminus binding
GO:0008094 DNA-dependent ATPase activity
GO:0008584 male gonad development
GO:0010165 response to X-ray
GO:0010224 response to UV-B
GO:0016446 somatic hypermutation of immunoglobulin genes
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0031573 intra-S DNA damage checkpoint
GO:0032137 guanine/thymine mispair binding
GO:0032301 MutSalpha complex
GO:0032302 MutSbeta complex
GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
GO:0042803 protein homodimerization activity
GO:0043524 negative regulation of neuron apoptosis
GO:0043570 maintenance of DNA repeat elements
GO:0045128 negative regulation of reciprocal meiotic recombination
GO:0045190 isotype switching
GO:0051096 positive regulation of helicase activity
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