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MSH2 antibody | 1B3A8A8

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Western Blot Validated
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to MSH2 (molecular weight 105 kDa)
Anti MSH2 Antibody, clone 1B3A8A8 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of control and MSH2 knockout eHAP whole cell lysates probed with MSH2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to MSH2 (molecular weight 105 kDa).

Mouse anti MSH2

Product Type
PrecisionAb Monoclonal
Clone
1B3A8A8
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00286
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human MSH2 antibody recognizes the DNA mismatch repair protein Msh2, also known as DNA mismatch repair protein Msh2, hMSH2, mutS homolog 2 and colon cancer, nonpolyposis type 1.

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for MSH2 (provided by RefSeq, Apr 2012).

Mouse anti Human MSH2 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MSH2 detects a band of approximately 105 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human MSH2 expressed in E. coli
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P43246
Entrez Gene
MSH2
GO Terms
GO:0000228 nuclear chromosome
GO:0000403 Y-form DNA binding
GO:0000404 loop DNA binding
GO:0000406 double-strand/single-strand DNA junction binding
GO:0000710 meiotic mismatch repair
GO:0005524 ATP binding
GO:0006301 postreplication repair
GO:0006302 double-strand break repair
GO:0006311 meiotic gene conversion
GO:0008022 protein C-terminus binding
GO:0008094 DNA-dependent ATPase activity
GO:0008584 male gonad development
GO:0010165 response to X-ray
GO:0010224 response to UV-B
GO:0016446 somatic hypermutation of immunoglobulin genes
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0031573 intra-S DNA damage checkpoint
GO:0032137 guanine/thymine mispair binding
GO:0032301 MutSalpha complex
GO:0032302 MutSbeta complex
GO:0042771 DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis
GO:0042803 protein homodimerization activity
GO:0043524 negative regulation of neuron apoptosis
GO:0043570 maintenance of DNA repeat elements
GO:0045128 negative regulation of reciprocal meiotic recombination
GO:0045190 isotype switching
GO:0051096 positive regulation of helicase activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MSH2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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