MRPL28 antibody | AB04/3B11

Mouse anti MRPL28

Product Type
PrecisionAb Monoclonal
Clone
AB04/3B11
Isotype
IgG1
Format
Purified
Specificity
MRPL28

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VMA00938
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WB 100 µl loader
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Mouse anti MRPL28 antibody recognizes 39S ribosomal protein L28, mitochondrial, also known as L28mt, MRP-L28, and melanoma antigen p15.

MRPL28 is a nuclearly encoded, mitochondrial protein required for correct function of the mitochindrial ribosome, and for synthesis of mitochondrially encoded proteins. Knockdown of MRPL28 in pancreatic cancer cells has been shown to decrease mitochondrial activity and inhibit cell growth (Chen et al. 2009). Hypermethylation of the MRPL28 gene locus has been suggested as a risk factor for asthma development (Gunawardhana et al. 2014). It has also been suggested that differences in the MRPL28 gene may play a role in obesity by altering fat oxidization (Lin et al. 2013).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti MRPL28 detects a band of approximately 28 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-256 of human MRPL28
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13084
Entrez Gene
MRPL28
GO Terms
GO:0003735 structural constituent of ribosome
GO:0005761 mitochondrial ribosome
GO:0006412 translation
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