Clone UCHT-1 recognises the human CD3 antigen, which is expressed by all mature T cells at the cell surface. CD3 is expressed in the cytoplasm of immature T cells.
Clone 2C7 recognises myeloperoxidase, an intracellular enzyme expressed by cells of myeloid lineage.
- Target Species
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
transmembrane receptor activity
T cell costimulation
positive thymic T cell selection
protein heterodimerization activity
T cell receptor signaling pathway
stored secretory granule
low-density lipoprotein particle remodeling
hydrogen peroxide catabolic process
receptor signaling protein activity
integral to plasma membrane
signal complex assembly
G-protein coupled receptor protein signaling pathway
external side of plasma membrane
SH3 domain binding
protein kinase binding
receptor signaling complex scaffold activity
T cell receptor binding
protein complex assembly
establishment or maintenance of cell polarity
regulation of apoptosis
T cell activation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
|Flow Cytometry 1
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Negative Isotype Controls Available
Useful Reagents Available
How to Use the Spectraviewer
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Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra