Mpo/CD3 | 2C7/UCHT1

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Mouse anti Human Myeloperoxidase:FITC/CD3:RPE

Product Type
Dual Color Reagent
Clone
2C7/UCHT1
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC031 F* 50 Tests
Clone UCHT-1 recognises the human CD3 antigen, which is expressed by all mature T cells at the cell surface. CD3 is expressed in the cytoplasm of immature T cells.

Clone 2C7 recognises myeloperoxidase, an intracellular enzyme expressed by cells of myeloid lineage.

Product Details

Target Species
Human
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG1 (MOUSE)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P09693 Related reagents
P05164 Related reagents
P04234 Related reagents
P07766 Related reagents
Entrez Gene
CD3D Related reagents
MPO Related reagents
CD3E Related reagents
CD3G Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005737 cytoplasm
GO:0031295 T cell costimulation
GO:0045059 positive thymic T cell selection
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0042608 T cell receptor binding
GO:0006461 protein complex assembly
GO:0007163 establishment or maintenance of cell polarity
GO:0015031 protein transport
GO:0042981 regulation of apoptosis
GO:0042110 T cell activation
Regulatory
For research purposes only

Applications of Mpo/CD3

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1:FITC/Mouse IgG1:RPE Negative Control DC012 50 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F