MMP-9 antibody | 4A3

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Mouse anti Human MMP-9 Activated

Product Type
Monoclonal Antibody
Clone
4A3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2736GA P WB 0.1 mg
MCA2736 P WB 0.2 mg
Mouse anti Human MMP-9 Activated antibody, clone 4A3 recognizes the active form of human matrix metalloproteinase 9 (MMP-9). The MMP’s are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP’s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-9 is a gelatinase, cleaving type IV collagen and gelatin. The gelatinases have an additional gelatin-binding domain inserted in the catalytic domain. MMP-9, alongside other MMPs, plays a role in normal tissue remodeling such as embryonic development, ovulation, mammary gland involution and wound healing. It is important in the early stages of tumor invasion as it degrades the type IV collagen in the basement membrane.

Mouse anti Human MMP-9 Activated antibody, clone 4A3 recognizes only the active form of MMP-9. It does not react with the MMP-9 proenzyme or the active or proenzyme forms of MMP-2.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Ovalbumin conjugated synthetic peptide corresponding to a region within the N-terminus of human MMP-9.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Ag8563 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P14780 Related reagents
Entrez Gene
MMP9 Related reagents
GO Terms
GO:0004222 metalloendopeptidase activity
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030225 macrophage differentiation
GO:0030574 collagen catabolic process
GO:0051549 positive regulation of keratinocyte migration
Regulatory
For research purposes only

Applications of MMP-9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/25 1/100
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Esophageal adenocarcinoma
Western Blotting
MCA2736 detects a band of approximately 63kDa.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for MMP-9 antibody

  1. Duncan, M.E. et al. (1998) Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
    Eur J Biochem. 258 (1): 37-43.
  2. Šelemetjev S et al. (2016) Coexpressed High Levels of VEGF-C and Active MMP-9 Are Associated With Lymphatic Spreading and Local Invasiveness of Papillary Thyroid Carcinoma.
    Am J Clin Pathol. Nov 2. pii: aqw184. [Epub ahead of print]