MMP-3 antibody | G05-6A10
Mouse anti Human MMP-3:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- G05-6A10
- Isotype
- IgG1
- Specificity
- MMP-3
| Mouse anti Human MMP-3 antibody, clone G05-6A10, recognizes matrix metalloproteinase-3 (MMP-3) also known as stromelysin-1 (SL-1) and transin-1. MMP-3 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets (Cui et al. 2017). MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form. Like other MMP’s, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin (Cui et al. 2017). Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents (Lech et al. 2019). The biotinylated Mouse anti Human MMP-3 antibody, clone G05-6A10 (MCA6259B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-3 antibody, clone I08-9G7 (MCA6258GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli derived recombinant human partial length of MMP-3 (Phe100-Cys477)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Capture Antibody:
MCA6258GA
ELISA Antigen-Positive Control:
PHP304
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human MMP-3 | MCA6258GA | E | 0.1 mg |
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| Description | Mouse anti Human MMP-3 | ||||||
| Recombinant Human MMP-3 | PHP304 | E | 10 µg | Log in | |||
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| Description | Recombinant Human MMP-3 | ||||||
- Synonyms
- Stromelysin-1
- UniProt
- P08254
- Entrez Gene
- MMP3
- GO Terms
- GO:0005509 calcium ion binding
- GO:0004222 metalloendopeptidase activity
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0030574 collagen catabolic process
- GO:0043231 intracellular membrane-bounded organelle
MCA6259B
100005531
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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