MMP-3 antibody | G05-6A10

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Mouse anti Human MMP-3:Biotin

Product Type
Monoclonal Antibody
Clone
G05-6A10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6259B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human MMP-3 antibody, clone G05-6A10, recognizes matrix metalloproteinase-3 (MMP-3) also known as stromelysin-1 (SL-1) and transin-1. MMP-3 is a member of the metzincins superfamily of proteases and is produced by various cells and tissues including the endothelium, vascular smooth muscle cells intima and platelets (Cui et al. 2017).

MMP-3 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the 57 kDa pro-MMP-3. This is then further cleaved by proteolytic enzymes to create the 45 kDa active form.

Like other MMP’s, MMP-3 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-3 degrades the collagen proteins, type II, IV, IX as well as the proteoglycans elastin fibronectin and laminin (Cui et al. 2017). Through the degradation of these ECM components, growth factors and adhesion molecules, MMP-3 can modify the extracellular environment and modulate cell to cell signaling. As such, MMP-3 is implicated in synaptic plasticity through enzymatic modification of ECM constituents (Lech et al. 2019).

The biotinylated Mouse anti Human MMP-3 antibody, clone G05-6A10 (MCA6259B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human MMP-3 antibody, clone I08-9G7 (MCA6258GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human partial length of MMP-3 (Phe100-Cys477)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P08254
Entrez Gene
MMP3
GO Terms
GO:0005509 calcium ion binding
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0043231 intracellular membrane-bounded organelle
Regulatory
For research purposes only

Applications of MMP-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human MMP-3 MCA6258GA E 0.1 mg loader
Recombinant Human MMP-3 PHP304 E 10 µg loader

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