MMP-3 antibody | 1B4

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Mouse anti Human MMP-3

Product Type
Monoclonal Antibody
Clone
1B4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2734GA E P* WB 0.1 mg
Mouse anti Human MMP-3 antibody, clone 1B4 recognizes human matrix metalloproteinase 3, also known as MMP-3, stromelysin-1 or transin-1. MMP-3 in its pro-form is a 477 amino acid ~54kDa zinc dependent endopeptidase processed to a 378 amino acid, ~45 kDa peptide in the mature form containing 4 hemopexin-like repeats.

MMPs are also involved in cell proliferation, migration, differentiation and apoptosis (Jana et al. 2012). Most MMP’s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis (Holmberg et al. 2013). MMP-3 can degrade proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen (Wilhelm et al. 1993). It can activate other MMP’s and may be involved in the repair processes of the heart after myocardial infarction (Wang et al. 2011).

Mouse anti human MMP-3 antibody, clone 1B4 has been used successfully for the identification of MMP-3 in gastric cancer by both Western blotting and immunohistochemistry (Murray et al. 1998)

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovalbumin conjugated synthetic peptide corresponding to a region within the C-terminus of human MMP-3.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Ag8563 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08254 Related reagents
Entrez Gene
MMP3 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0043231 intracellular membrane-bounded organelle
Regulatory
For research purposes only

Applications of MMP-3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Gastric carinoma
Western Blotting
MCA2734GA detects a band of approximately 50kDa in cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for MMP-3 antibody

  1. Murray, G.I. et al. (1998) Matrix metalloproteinases and their inhibitors in gastric cancer.
    Gut. 43 (6): 791-7.
  2. Lyall, M.S. et al. (2006) Profiling markers of prognosis in colorectal cancer.
    Clin Cancer Res. 12 (4): 1184-91.