Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The MMP2 gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in MMP-2 have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for MMP2 (provided by RefSeq, Jul 2008).
Mouse anti Human MMP-2 antibody detects a band of 74 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti MMP-2 detects a band of approximately 74 kDa in HeLa cell lysates.
- Product Form
- Purified IgG - liquid
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Purified recombinant fragment of human MMP-2, amino acids 242-396, expressed in E. coli
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P08253 Related reagents
- Entrez Gene
- MMP2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0001525 angiogenesis
- GO:0004222 metalloendopeptidase activity
- GO:0005578 proteinaceous extracellular matrix
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0006508 proteolysis
- GO:0008270 zinc ion binding
- GO:0030574 collagen catabolic process
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of MMP-2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||WB||2 ml|
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