MLH1 antibody | UMAB191

Mouse anti MLH1

Product Type
PrecisionAb Monoclonal
Clone
UMAB191
Isotype
IgG1
Format
Purified
Specificity
MLH1

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VMA00385
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Mouse anti Human MLH1 antibody, clone UMAB191 recognizes the DNA mismatch repair protein MLH1, also known as mutL homolog 1 and colon cancer, nonpolyposis type 2.

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined (provided by RefSeq, Nov 2009).

Mouse anti Human MLH1 antibody detects a band of 85 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MLH1 detects a band of approximately 85 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 299-527 of human MLH1 (NP_000240) produced in E.coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P40692
Entrez Gene
MLH1
GO Terms
GO:0005515 protein binding
GO:0000795 synaptonemal complex
GO:0005524 ATP binding
GO:0005712 chiasma
GO:0006298 mismatch repair
GO:0016887 ATPase activity
GO:0016446 somatic hypermutation of immunoglobulin genes
GO:0032389 MutLalpha complex
GO:0032390 MutLbeta complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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