MIF antibody | 2A10-4D3

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Anti Human MIF Antibody, clone 2A10-4D3 gallery image 1 — Staining of paraffin embedded human lung adenosquamous cell carcinoma with Mouse anti Human MIF:Azide Free (MCA3263Z)

Mouse anti Human MIF:Preservative Free

Product Type: Monoclonal Antibody
Clone: 2A10-4D3
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA3263Z	P*			0.1 mg

Mouse anti Human MIF antibody, clone 2A10-4D3 recognizes human Macrophage migration inhibitory factor, also known as MIF, glycosylation-inhibiting factor, L-dopachrome isomerase or phenylpyruvate tautomerase. MIF is a 115 amino acid ~12.5 kDa pro-inflammatory cytokine playing a role in the innate immune response to bacterial pathogens. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids (UniProt:14174). MIF and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways (Kleemann et al. 2000). Mutation of the MIF gene is implicated in susceptibility and severity of early onset rheumatoid arthritis (Llamas-Covarrubias et al. 2013).

Mouse anti Human MIF antibody, clone 2A10-4D3 has been utilized successfully for the micro immunoaffinity isolation of human MIF from human serum for subsequent mass spectormetric immunoassay (Sherma 2014).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant protein with a GST tag corresponding to amino acids 1 - 116 of human MIF.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P14174 Related reagents
Entrez Gene: MIF Related reagents
GO Terms: GO:0001516 prostaglandin biosynthetic process; GO:0004167 dopachrome isomerase activity; GO:0005125 cytokine activity; GO:0005126 cytokine receptor binding; GO:0005615 extracellular space; GO:0005737 cytoplasm; GO:0006954 inflammatory response; GO:0007166 cell surface receptor linked signaling pathway; GO:0008283 cell proliferation; GO:0009986 cell surface; GO:0010629 negative regulation of gene expression; GO:0010739 positive regulation of protein kinase A signaling cascade; GO:0030890 positive regulation of B cell proliferation; GO:0033138 positive regulation of peptidyl-serine phosphorylation; GO:0042056 chemoattractant activity; GO:0043030 regulation of macrophage activation; GO:0043066 negative regulation of apoptosis; GO:0043498 cell surface binding; GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator; GO:0045087 innate immune response; GO:0048146 positive regulation of fibroblast proliferation; GO:0050178 phenylpyruvate tautomerase activity; GO:0050715 positive regulation of cytokine secretion; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0070207 protein homotrimerization; GO:0070374 positive regulation of ERK1 and ERK2 cascade; GO:0071157 negative regulation of cell cycle arrest
Regulatory: For research purposes only

Applications of MIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹		0.1	10 ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for MIF antibody

Sherma, N.D. et al. (2014) Mass Spectrometric Immunoassay for the qualitative and quantitative analysis of the cytokine Macrophage Migration Inhibitory Factor (MIF).
Proteome Sci. 12 (1): 52.

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