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MIF antibody | 2A10-4D3

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Anti Human MIF Antibody, clone 2A10-4D3 gallery image 1
Staining of paraffin embedded human lung adenosquamous cell carcinoma with Mouse anti Human MIF:Azide Free (MCA3263Z)

Mouse anti Human MIF:Preservative Free

Product Type
Monoclonal Antibody
Clone
2A10-4D3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA3263Z
Datasheet
P*
SDS
0.1 mg loader

Mouse anti Human MIF antibody, clone 2A10-4D3 recognizes human Macrophage migration inhibitory factor, also known as MIF, glycosylation-inhibiting factor, L-dopachrome isomerase or phenylpyruvate tautomerase. MIF is a 115 amino acid ~12.5 kDa pro-inflammatory cytokine playing a role in the innate immune response to bacterial pathogens. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids (UniProt:14174). MIF and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways (Kleemann et al. 2000). Mutation of the MIF gene is implicated in susceptibility and severity of early onset rheumatoid arthritis (Llamas-Covarrubias et al. 2013).

Mouse anti Human MIF antibody, clone 2A10-4D3 has been utilized successfully for the micro immunoaffinity isolation of human MIF from human serum for subsequent mass spectormetric immunoassay (Sherma 2014).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein with a GST tag corresponding to amino acids 1 - 116 of human MIF.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P14174
Entrez Gene
MIF
GO Terms
GO:0001516 prostaglandin biosynthetic process
GO:0004167 dopachrome isomerase activity
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0010629 negative regulation of gene expression
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0030890 positive regulation of B cell proliferation
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042056 chemoattractant activity
GO:0043030 regulation of macrophage activation
GO:0043066 negative regulation of apoptosis
GO:0043498 cell surface binding
GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045087 innate immune response
GO:0048146 positive regulation of fibroblast proliferation
GO:0050178 phenylpyruvate tautomerase activity
GO:0050715 positive regulation of cytokine secretion
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0070207 protein homotrimerization
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071157 negative regulation of cell cycle arrest
Regulatory
For research purposes only

Applications of MIF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for MIF antibody

  1. Sherma, N.D. et al. (2014) Mass Spectrometric Immunoassay for the qualitative and quantitative analysis of the cytokine Macrophage Migration Inhibitory Factor (MIF).
    Proteome Sci. 12 (1): 52.

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