MGMT antibody | UMAB56

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Anti MGMT Antibody, clone UMAB56 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MGMT Antibody, clone UMAB56 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MGMT antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to MGMT (molecular weight 23 kDa).

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Mouse anti MGMT

Product Type: PrecisionAb Monoclonal
Clone: UMAB56
Isotype: IgG1
Format: Purified
Specificity: MGMT

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VMA00426 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human MGMT antibody, clone UMAB56 recognizes methylguanine-DNA methyltransferase (MGMT), also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase and methylated-DNA--protein-cysteine methyltransferase.

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by the MGMT gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma (provided by RefSeq, Sep 2015).

Mouse anti Human MGMT antibody, clone UMAB56 detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MGMT detects a band of approximately 23 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human MGMT (NP_002403) produced in E.coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P16455
Entrez Gene: MGMT
GO Terms: GO:0003677 DNA binding; GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity; GO:0046872 metal ion binding; GO:0005654 nucleoplasm; GO:0006266 DNA ligation; GO:0009008 DNA-methyltransferase activity