MGMT antibody | UMAB56

Mouse anti MGMT

Product Type
PrecisionAb Monoclonal
Clone
UMAB56
Isotype
IgG1
Format
Purified
Specificity
MGMT

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VMA00426
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Mouse anti Human MGMT antibody, clone UMAB56 recognizes methylguanine-DNA methyltransferase (MGMT), also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase and methylated-DNA--protein-cysteine methyltransferase.

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by the MGMT gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma (provided by RefSeq, Sep 2015).

Mouse anti Human MGMT antibody, clone UMAB56 detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MGMT detects a band of approximately 23 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human MGMT (NP_002403) produced in E.coli
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P16455
Entrez Gene
MGMT
GO Terms
GO:0003677 DNA binding
GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0006266 DNA ligation
GO:0009008 DNA-methyltransferase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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