MGMT antibody | UMAB56

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Mouse anti MGMT

Mouse anti human MGMT antibody recognizes methylated-DNA-protein-cysteine methyltransferase, also known as 6-O-methylguanine-DNA methyltransferase, or methylguanine-DNA methyltransferase. This antibody has been extensively validated for western blotting using whole cell lysates.

MGMT Antibody with Control Lysate

Mouse anti human MGMT antibody recognizes methylated-DNA-protein-cysteine methyltransferase, also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase or methylguanine-DNA methyltransferase.

Product Type
PrecisionAb™ Monoclonal
Clone
UMAB56
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00426 WB 100 µl
VMA00426KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human MGMT antibody recognizes methylguanine-DNA methyltransferase (MGMT), also known as 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase and methylated-DNA--protein-cysteine methyltransferase.

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by the MGMT gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma (provided by RefSeq, Sep 2015).

Mouse anti Human MGMT antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MGMT detects a band of approximately 23 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human MGMT (NP_002403) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P16455 Related reagents
Entrez Gene
MGMT Related reagents
GO Terms
GO:0003677 DNA binding
GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0006266 DNA ligation
GO:0009008 DNA-methyltransferase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of MGMT antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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