Find out more about A.I.den - our SearchBot

MELAN-A antibody | M2-7C10

100% Secure
No associated image currently available
No associated image currently available

Mouse anti Human MELAN-A

Product Type
Monoclonal Antibody
Clone
M2-7C10
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2207
Datasheet
P*
MSDS
1 ml loader

Mouse anti Human MELAN-A antibody, clone M2-7C10 recognizes MELAN-A/MART-1, which is a melanocyte differentiation marker.

MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines.

MELAN-A/MART-1 is not expressed by non-melanoma malignancies.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
1.0%Bovine Serum Albumin
Immunogen
MELAN-A fusion protein.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended.
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
Q16655
Entrez Gene
MLANA
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005802 trans-Golgi network
GO:0042470 melanosome
Regulatory
For research purposes only

Applications of MELAN-A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/40
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. STUF MARK2 (UNIVERSAL) BUF025A/B is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Histology Positive Control Tissue
Melanoma
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for MELAN-A antibody

  1. Marincola, F.M. et al. (1996) Analysis of expression of the melanoma-associated antigens MART-1 and gp100 in metastatic melanoma cell lines and in in situ lesions.
    J Immunother Emphasis Tumor Immunol. 19 (3): 192-205.
  2. Fetsch, P.A. et al. (2001) Melanoma antigen expression in serial fine-needle aspiration samples in patients with metastatic malignant melanoma participating in immunotherapy clinical trials: a preliminary look.
    Cancer. 93 (6): 409-14.
  3. Win, S.J. et al. (2011) Cross-presentation of epitopes on virus-like particles via the MHC I receptor recycling pathway.
    Immunol Cell Biol. 89: 681-8.
  4. Win, S.J. et al. (2012) Enhancing the immunogenicity of tumour lysate-loaded dendritic cell vaccines by conjugation to virus-like particles.
    Br J Cancer. 106 (1): 92-8.
Request a different product with the MELAN-A specificity