MELAN-A antibody | M2-7C10
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Mouse anti Human MELAN-A antibody, clone M2-7C10 recognizes MELAN-A/MART-1, which is a melanocyte differentiation marker.
MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines.
MELAN-A/MART-1 is not expressed by non-melanoma malignancies.
MELAN-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines.
MELAN-A/MART-1 is not expressed by non-melanoma malignancies.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 1.0% Bovine Serum Albumin - Immunogen
- MELAN-A fusion protein.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- Q16655
- Entrez Gene
- MLANA
- GO Terms
- GO:0005515 protein binding
- GO:0005887 integral to plasma membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0005802 trans-Golgi network
- GO:0042470 melanosome
- Regulatory
- For research purposes only
Applications of MELAN-A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 1/40 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. STUF MARK2 (UNIVERSAL) BUF025A/B is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Melanoma
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for MELAN-A antibody
-
Marincola, F.M. et al. (1996) Analysis of expression of the melanoma-associated antigens MART-1 and gp100 in metastatic melanoma cell lines and in in situ lesions.
J Immunother Emphasis Tumor Immunol. 19 (3): 192-205. -
Fetsch, P.A. et al. (2001) Melanoma antigen expression in serial fine-needle aspiration samples in patients with metastatic malignant melanoma participating in immunotherapy clinical trials: a preliminary look.
Cancer. 93 (6): 409-14. -
Win, S.J. et al. (2011) Cross-presentation of epitopes on virus-like particles via the MHC I receptor recycling pathway.
Immunol Cell Biol. 89: 681-8. -
Win, S.J. et al. (2012) Enhancing the immunogenicity of tumour lysate-loaded dendritic cell vaccines by conjugation to virus-like particles.
Br J Cancer. 106 (1): 92-8.
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