MEK 1 antibody

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Human anti MEK 1

MEK 1 antibody recognizes dual specificity mitogen-activated protein kinase kinase 1, also known as MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1 or MEK 1. The antibody has been extensively validated for western blotting using whole cell lysates.

MEK 1 Antibody with Control Lysate

MEK 1 antibody recognizes dual specificity mitogen-activated protein kinase kinase 1, also known as MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1 or MEK 1. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00010 WB 100 µl
VMA00010KT WB 2 Westerns
With Lysate 400µg
Human anti Human MEK 1 antibody recognizes human dual specificity mitogen-activated protein kinase kinase 1, also known as MEK 1 or MAPKK1. MEK 1 is a 393 amino acid, dual specificity protein kinase which has the ability to phosphorylate both threonine and tyrosine residues. MEK1 acts downstream of Ras and Raf and is responsible for the phosphorylation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK). It is an integral component of the MAP kinase signalling cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.

Human anti Human MEK 1 antibody recognizes human MEK1 as a band of ~42 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti MEK 1 antibody detects a band of approximately 42 kDa in Jurkat cell lysates.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
Preparation
Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Preparation
20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Full length recombinant human MEK 1 expressed in Sf9 cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q02750 Related reagents
Entrez Gene
MAP2K1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004708 MAP kinase kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
GO:0090398 cellular senescence
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories. His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of MEK 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB
Goat anti Human IgG F(ab')2:HRP STAR126P 0.5 ml E WB

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