MEK 1 antibody | AbD05812

100% Secure


Human anti Human MEK 1

Product Type
Monoclonal Antibody
Clone
AbD05812
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA067 E WB 0.1 mg
Human anti Human MEK 1 antibody, clone AbD05812 recognizes human dual specificity mitogen-activated protein kinase kinase 1, also known, MEK 1 or MAPKK1. MEK 1 is a 393 amino acid ~43 kDa dual specificity protein kinase which has the ability to phosphorylate both threonine and tyrosine residues. MEK1 acts downstream of Ras and Raf and is responsible for the phosphorylation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK). It is an integral component of the MAP kinase signaling cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Full length recombinant human MEK 1 expressed in Sf9 cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q02750 Related reagents
Entrez Gene
MAP2K1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0004708 MAP kinase kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
GO:0090398 cellular senescence
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of MEK 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/100 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Western Blotting
HCA067 detects a band of approximately 43 kDa in HCT-116 cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB