MCM4 antibody | AB03/1F7

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siRNA Validated

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MCM4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to MCM4 (molecular weight 101 kDa)

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MCM4 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti MCM4 antibody (**VMA00771**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 9 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti MCM4 Antibody, clone AB03/1F7 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of MCM4 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MCM4 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti MCM4

Product Type: PrecisionAb Monoclonal
Clone: AB03/1F7
Isotype: IgG2b
Format: Purified
Specificity: MCM4

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00771 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human MCM4 antibody recognizes the DNA replication licensing factor MCM4.

The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. MCM4 is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported (provided by RefSeq, Jul 2008).

Mouse anti MCM4 antibody detects a band of 101 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Mouse anti MCM4 detects a band of approximately 101 kDa in MOLT-4 cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 638-863 of human MCM4
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P33991
Entrez Gene: MCM4
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0003677 DNA binding; GO:0017111 nucleoside-triphosphatase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; View More GO Terms; GO:0006270 DNA-dependent DNA replication initiation; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006350 transcription; GO:0045449 regulation of transcription