Mouse anti MCM3
Mouse anti-human MCM3 antibody recognizes DNA replication licensing factor MCM3, also known as DNA polymerase alpha holoenzyme-associated protein P1, MCM3 minichromosome maintenance deficient 3, or RLF subunit beta.
- Product Type
- PrecisionAb™ Monoclonal
|Product Code||Applications||Datasheet||MSDS||Pack Size||List Price||Quantity|
The protein encoded by MCM3 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for MCM3 (provided by RefSeq, Jul 2012).
Mouse anti Human MCM3 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti MCM3 detects a band of approximately 105 kDa in Jurkat cell lysate
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Recombinant protein of human MCM3
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P25205 Related reagents
- Entrez Gene
- MCM3 Related reagents
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0003677 DNA binding
- GO:0017111 nucleoside-triphosphatase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005658 alpha DNA polymerase:primase complex
- GO:0005813 centrosome
- GO:0006270 DNA-dependent DNA replication initiation
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006350 transcription
- GO:0045449 regulation of transcription
- GO:0048471 perinuclear region of cytoplasm
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of MCM3 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Mouse IgG (H/L):HRP||STAR207P||2 ml||WB|
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.