MCM3 antibody

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Mouse anti MCM3

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00487
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human MCM3 antibody recognizes the DNA replication licensing factor MCM3, also known as DNA polymerase alpha holoenzyme-associated protein P1, MCM3 minichromosome maintenance deficient 3, cervical cancer proto-oncogene 5 or replication licensing factor, beta subunit.

The protein encoded by MCM3 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for MCM3 (provided by RefSeq, Jul 2012).

Mouse anti Human MCM3 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MCM3 detects a band of approximately 105 kDa in Jurkat cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein of human MCM3

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P25205 Related reagents
Entrez Gene
MCM3 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0003677 DNA binding
GO:0017111 nucleoside-triphosphatase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005658 alpha DNA polymerase:primase complex
GO:0005813 centrosome
GO:0006270 DNA-dependent DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006350 transcription
GO:0045449 regulation of transcription
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MCM3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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