MCM2 antibody | OTI3B6

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Anti MCM2 Antibody, clone OTI3B6 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MCM2 Antibody, clone OTI3B6 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with MCM2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to MCM2 (molecular weight 125 kDa)

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Mouse anti MCM2

Product Type: PrecisionAb Monoclonal
Clone: OTI3B6
Isotype: IgG1
Format: Purified
Specificity: MCM2

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VMA00259 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human MCM2 antibody recognizes the DNA replication licensing factor MCM2, also known as DNA replication licensing factor MCM2, MCM2 minichromosome maintenance deficient 2, mitotin, cell devision cycle-like 1, cyclin-like 1, minichromosome maintenance deficient 2 (mitotin), minichromosome maintenance protein 2 homolog and nuclear protein BM28.

The protein encoded by MCM2 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined (provided by RefSeq, Oct 2012).

Mouse anti Human MCM2 antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti MCM2 detects a band of approximately 125 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human MCM2 (NP_004517) produced in HEK293T cells
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P49736
Entrez Gene: MCM2
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000785 chromatin; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0005654 nucleoplasm; View More GO Terms; GO:0006270 DNA-dependent DNA replication initiation; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006350 transcription; GO:0045449 regulation of transcription