Mast Cell Chymase antibody | CC1

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Mouse anti Human Mast Cell Chymase

Product Type: Monoclonal Antibody
Clone: CC1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1930T	C P			10 µg
MCA1930	C E P WB			0.1 mg

Mouse anti Human Mast Cell Chymase antibody, clone CC1 binds to human mast cell chymase, an important marker of mast cells and also an important mediator of inflammation.

Mouse anti Human Mast Cell Chymase antibody, clone CC1 may be used to detect mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart.

Binding of Mouse anti Human Mast Cell Chymase antibody, clone CC1 to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Purified human skin chymase.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the P3/NSI/I.Ag4.1 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P23946 Related reagents
Entrez Gene: CMA1 Related reagents
GO Terms: GO:0004252 serine-type endopeptidase activity; GO:0005576 extracellular region; GO:0006508 proteolysis; GO:0050720 interleukin-1 beta biosynthetic process; GO:0050727 regulation of inflammatory response
Regulatory: For research purposes only

Applications of Mast Cell Chymase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin	1/500	1ug/ml Pack Size: 10 µg 1/1000 Pack Size: 0.1 mg
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue: Skin

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

ELISA

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

Source Reference

Buckley, M.G. et al. (1999) The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase.
J Pathol. 189 (1): 138-43.

References for Mast Cell Chymase antibody

Mauro, L.V. et al. (2008) Association between mast cells of different phenotypes and angiogenesis in colorectal cancer.
Mol Med Rep. 1 (6): 895-902.
Dougherty, R.H. et al. (2010) Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma.
J Allergy Clin Immunol. 125 (5): 1046-1053.e8.
Xiang, M. et al. (2011) Usefulness of serum tryptase level as an independent biomarker for coronary plaque instability in a Chinese population.
Atherosclerosis. 215 (2): 494-9.
Sun, J. et al. (2009) Critical role of mast cell chymase in mouse abdominal aortic aneurysm formation.
Circulation. 120: 973-82.
Laidlaw, T.M. et al. (2011) Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI.
J Allergy Clin Immunol. 127: 815-22.e1-5.
Lin, A.M. et al. (2011) Mast Cells and Neutrophils Release IL-17 through Extracellular Trap Formation in Psoriasis.
J Immunol. 187: 490-500.
Veerappan, A. et al. (2012) Mast cells are required for the development of renal fibrosis in the rodent unilateral ureteral obstruction model.
Am J Physiol Renal Physiol. 302 (1): F192-204.
Buckley, M. Walls, A.F. (2008) Identification of mast cells and mast cell subpopulations.
Methods Mol Med. 138: 285-97.
Bhattacharya, S. et al. (2012) Genome-wide transcriptional profiling reveals connective tissue mast cell accumulation in bronchopulmonary dysplasia.
Am J Respir Crit Care Med. 186: 349-58.
Martin, L.J. et al. (2015) Pediatric Eosinophilic Esophagitis Symptom Scores (PEESS v2.0) identify histologic and molecular correlates of the key clinical features of disease.
J Allergy Clin Immunol. 135 (6): 1519-28.e8.
Badertscher, K. et al. (2005) Mast cell chymase is increased in chronic atopic dermatitis but not in psoriasis.
Arch Dermatol Res. 296 (10): 503-6.
Allam, J.P. et al. (2008) Distribution of Langerhans cells and mast cells within the human oral mucosa: new application sites of allergens in sublingual immunotherapy?
Allergy. 63 (6): 720-7.
Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
Clin Exp Allergy. 37 (11): 1648-56.
Lipitsä, T. et al. (2015) Mast cell tryptase and chymase in the progress of cutaneous vasculitis.
Arch Dermatol Res. 307 (10): 917-24.
Suttle, M.M. & Harvima, I.T. (2016) Mast cell chymase in experimentally induced psoriasis.
J Dermatol. 43 (6): 693-6.

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