Mast Cell Chymase antibody | CC1

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Mouse anti Human Mast Cell Chymase

Product Type
Monoclonal Antibody
Clone
CC1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1930T C P 10 µg
MCA1930 C E P WB 0.1 mg
Mouse anti Human Mast Cell Chymase antibody, clone CC1 binds to human mast cell chymase, an important marker of mast cells and also an important mediator of inflammation.

Mouse anti Human Mast Cell Chymase antibody, clone CC1 may be used to detect mast cells in routinely fixed tissues, and has been used to study the distribution of mast cells in skin, synovium, lung and heart.

Binding of Mouse anti Human Mast Cell Chymase antibody, clone CC1 to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified human skin chymase.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the P3/NSI/I.Ag4.1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P23946 Related reagents
Entrez Gene
CMA1 Related reagents
GO Terms
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0050720 interleukin-1 beta biosynthetic process
GO:0050727 regulation of inflammatory response
Regulatory
For research purposes only

Applications of Mast Cell Chymase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1/500 1ug/ml Pack Size: 10 µg
1/1000 Pack Size: 0.1 mg
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Skin

Application Based External Images

ELISA

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

Source Reference

  1. Buckley, M.G. et al. (1999) The detection of mast cell subpopulations in formalin-fixed human tissues using a new monoclonal antibody specific for chymase.
    J Pathol. 189 (1): 138-43.

References for Mast Cell Chymase antibody

  1. Mauro, L.V. et al. (2008) Association between mast cells of different phenotypes and angiogenesis in colorectal cancer.
    Mol Med Rep. 1 (6): 895-902.
  2. Dougherty, R.H. et al. (2010) Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma.
    J Allergy Clin Immunol. 125 (5): 1046-1053.e8.
  3. Xiang, M. et al. (2011) Usefulness of serum tryptase level as an independent biomarker for coronary plaque instability in a Chinese population.
    Atherosclerosis. 215 (2): 494-9.
  4. Sun, J. et al. (2009) Critical role of mast cell chymase in mouse abdominal aortic aneurysm formation.
    Circulation. 120: 973-82.
  5. Laidlaw, T.M. et al. (2011) Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI.
    J Allergy Clin Immunol. 127: 815-22.e1-5.
  6. Lin, A.M. et al. (2011) Mast Cells and Neutrophils Release IL-17 through Extracellular Trap Formation in Psoriasis.
    J Immunol. 187: 490-500.
  7. Veerappan, A. et al. (2012) Mast cells are required for the development of renal fibrosis in the rodent unilateral ureteral obstruction model.
    Am J Physiol Renal Physiol. 302 (1): F192-204.
  8. Buckley, M. Walls, A.F. (2008) Identification of mast cells and mast cell subpopulations.
    Methods Mol Med. 138: 285-97.
  9. Bhattacharya, S. et al. (2012) Genome-wide transcriptional profiling reveals connective tissue mast cell accumulation in bronchopulmonary dysplasia.
    Am J Respir Crit Care Med. 186: 349-58.
  10. Martin, L.J. et al. (2015) Pediatric Eosinophilic Esophagitis Symptom Scores (PEESS v2.0) identify histologic and molecular correlates of the key clinical features of disease.
    J Allergy Clin Immunol. 135 (6): 1519-28.e8.
  11. Badertscher, K. et al. (2005) Mast cell chymase is increased in chronic atopic dermatitis but not in psoriasis.
    Arch Dermatol Res. 296 (10): 503-6.
  12. Allam, J.P. et al. (2008) Distribution of Langerhans cells and mast cells within the human oral mucosa: new application sites of allergens in sublingual immunotherapy?
    Allergy. 63 (6): 720-7.
  13. Galatowicz, G. et al. (2007) Ocular anti-allergic compounds selectively inhibit human mast cell cytokines in vitro and conjunctival cell infiltration in vivo.
    Clin Exp Allergy. 37 (11): 1648-56.
  14. Lipitsä, T. et al. (2015) Mast cell tryptase and chymase in the progress of cutaneous vasculitis.
    Arch Dermatol Res. 307 (10): 917-24.
  15. Suttle, M.M. & Harvima, I.T. (2016) Mast cell chymase in experimentally induced psoriasis.
    J Dermatol. 43 (6): 693-6.