MAPK8/JNK1 (pThr183/pTyr185) antibody | D04/7G6

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Mouse anti MAPK8/JNK1 (pThr183/pTyr185)

MAPK8/JNK1 (pThr183/pTyr185) antibody recognizes mitogen-activated protein kinase 8 when phosphorylated at threonine 183 and tyrosine 185.

Product Type
PrecisionAb Monoclonal
Clone
D04/7G6
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00714
Datasheet
WB
SDS
100 µl loader

Mouse anti Human MAPK8/JNK1 (pThr183/pTyr185) antibody recognizes mitogen-activated protein kinase 8, also known as JUN N-terminal kinase, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta 2 and stress-activated protein kinase 1c, when phosphorylated at threonine 183 and tyrosine 187.

Encoded by the MAPK8/JNK1 gene, mitogen-activated protein kinase 8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of MAPK8 suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation (provided by RefSeq, Jun 2013). Activation of MAPK8/JNK1 requires phosphorylation at threonine 183 and tyrosine 185 (Zheng et al. 1999.

Mouse anti Human MAPK8/JNK1 (pThr183/pTyr185) antibody detects a band of 44/52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Mouse anti MAPK8/JNK1 (pThr183/pTyr185) detects bands of approximately 44 & 52 kDa in UV-treated HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the phosphorylated sites
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P45983
Entrez Gene
MAPK8
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004705 JUN kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0009411 response to UV
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043066 negative regulation of apoptosis
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of MAPK8/JNK1 (pThr183/pTyr185) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml