MAPK8/JNK1 (pThr183/pTyr185) antibody | D04/7G6
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WB ResetMouse anti MAPK8/JNK1 (pThr183/pTyr185)
- Product Type
- PrecisionAb Monoclonal
- Clone
- D04/7G6
- Isotype
- IgG1
- Format
- Purified
- Specificity
- MAPK8/JNK1 (pThr183/pTyr185)
Antibody Quality and Performance Guarantee
| Mouse anti Human MAPK8/JNK1 (pThr183/pTyr185) antibody recognizes mitogen-activated protein kinase 8, also known as JUN N-terminal kinase, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta 2 and stress-activated protein kinase 1c, when phosphorylated at threonine 183 and tyrosine 187. Encoded by the MAPK8/JNK1 gene, mitogen-activated protein kinase 8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. The mouse counterpart of MAPK8 suggests that this kinase plays a key role in T cell proliferation, apoptosis and differentiation (provided by RefSeq, Jun 2013). Activation of MAPK8/JNK1 requires phosphorylation at threonine 183 and tyrosine 185 (Zheng et al. 1999). Mouse anti Human MAPK8/JNK1 (pThr183/pTyr185) antibody detects a band of 44/52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Mouse anti MAPK8/JNK1 (pThr183/pTyr185) detects bands of approximately 44 & 52 kDa in UV-treated HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the phosphorylated sites
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
1/500 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
Further Reading
-
Sehgal, V. & Ram, P.T. (2013) Network Motifs in JNK Signaling.
Genes Cancer. 4 (9-10): 409-13.
- UniProt
- P45983
- Entrez Gene
- MAPK8
- GO Terms
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0005829 cytosol
- GO:0004705 JUN kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0008063 Toll signaling pathway
- GO:0008629 induction of apoptosis by intracellular signals
- View More GO Terms
- GO:0008633 activation of pro-apoptotic gene products
- GO:0009411 response to UV
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043066 negative regulation of apoptosis
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051090 regulation of transcription factor activity
- GO:0051403 stress-activated MAPK cascade
VMA00714
100004600
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