MAP3K13 antibody | 4H7

Mouse anti Human MAP3K13:Preservative Free

Product Type
Monoclonal Antibody
Clone
4H7
Isotype
IgG2a
Specificity
MAP3K13

Product Code Applications Pack Size List Price Your Price Qty
MCA5033Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human MAP3K13 antibody, clone 4H7 recognizes human mitogen activated protein kinase kinase kinase 13, also known as MAP3K13, Leucine zipper-bearing kinase or Mixed lineage kinase. MAP3K13 is a 966 amino acid ~120 kDa kinase involved in Activation of the JUN N-terminal pathway.

Five isoforms of MAP3K13 generated by alternative splicing have been described. Mouse anti Human MAP3K13 antibody, clone 4H7 binds to the canonical isoform 1. The antibody was generated using an immunogen corresponding to sequence toward the c-terminal region of MAP3K13 and is expected to also recognize isoforms 3 and 4 in which the c-terminal region is unaltered. However Mouse anti Human MAP3K13 antibody, clone 4H7 is not expected to recognize potential isoforms 2 and 5 both of which have deletions of the c-terminal region including the complete immunogen sequence used to isolate clone 4H7 (UniProt : O43283).

MAP3K13 is strongly expressed in the pancreas, it is also expressed in adult brain, liver and placenta as assessed using Northern blot analysis (Sakuma et al.1997).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 711-810 of human MAP3K13.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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RRID
AB_2141856
UniProt
O43283
Entrez Gene
MAP3K13
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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