MALT1 antibody
Mouse anti MALT1
MALT1 antibody recognizes mucosa-associated lymphoid tissue lymphoma translocation protein 1, also known as MALT lymphoma-associated translocation or paracaspase. The antibody has been extensively validated for western blotting using whole cell lysates.
MALT1 Antibody with Control Lysate
MALT1 antibody recognizes mucosa-associated lymphoid tissue lymphoma translocation protein 1, also known as MALT lymphoma-associated translocation or paracaspase. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00038 | WB | 100 µl | ||||
| VMA00038KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human MALT1 antibody recognizes human mucosal-associated lymphoid tissue lymphoma translocation 1 (MALT1), also known as MALT lymphoma-associated translocation and paracaspase. MALT1 is an 824 amino acid protein belonging to the peptidase C14B family containing a single death domain and two Ig-like C2-type domains. In normal lymphocytes, MALT1 plays an important role in antigen receptor-mediated lymphocyte activation. In T-cells MALT1 is recruited by activated CARMA1, along with Bcl-10, to form a CARMA1-Bcl10-MALT1 (CBM) complex which is involved in the activation of NF-kappaB (Yang et al. 2013).
MALT1 was initially identified as a recurrent translocation t(11;18)(q21:q21) in mucosal-associated lymphomas (Horsman et al. 1992), which creates a functional fusion oncoprotein consisting of MALT1 and apoptosis inhibitor API2 (Dierlamm et al. 1999). Studies suggest that overexpression of MALT1, or expression of the API2-MALT1 fusion protein, leads to the uncontrolled activation of NF-kappaB which is stimulus-independent (Zhou et al. 2005). Mouse anti Human MALT1 recognizes both MALT1 and the MALT1-API2 fusion protein (Rosebeck et al.2011, Murga Penas et al. 2007). The MALT1-API2 oncoprotein plays a role in non-canonical activation of NF-κb through NIK cleavage.
Mouse anti Human MALT1 antibody recognizes human MALT1 as a single band of ~92 kDa in multiple human cell line lysates by western blotting under reducing conditions.
MALT1 was initially identified as a recurrent translocation t(11;18)(q21:q21) in mucosal-associated lymphomas (Horsman et al. 1992), which creates a functional fusion oncoprotein consisting of MALT1 and apoptosis inhibitor API2 (Dierlamm et al. 1999). Studies suggest that overexpression of MALT1, or expression of the API2-MALT1 fusion protein, leads to the uncontrolled activation of NF-kappaB which is stimulus-independent (Zhou et al. 2005). Mouse anti Human MALT1 recognizes both MALT1 and the MALT1-API2 fusion protein (Rosebeck et al.2011, Murga Penas et al. 2007). The MALT1-API2 oncoprotein plays a role in non-canonical activation of NF-κb through NIK cleavage.
Mouse anti Human MALT1 antibody recognizes human MALT1 as a single band of ~92 kDa in multiple human cell line lysates by western blotting under reducing conditions.
Product Details
- Target Species
- Human
- Western Blotting
- Anti MALT1 antibody detects a band of approximately 92 kDa in HEK293 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein corresponding to amino acids 701-808 located within the C-terminus of human MALT1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9UDY8 Related reagents
- Entrez Gene
- MALT1 Related reagents
- GO Terms
- GO:0004197 cysteine-type endopeptidase activity
- GO:0002726 positive regulation of T cell cytokine production
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0006508 proteolysis
- GO:0006916 anti-apoptosis
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0032449 CBM complex
- GO:0032743 positive regulation of interleukin-2 production
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043621 protein self-association
- GO:0048471 perinuclear region of cytoplasm
- GO:0050852 T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051168 nuclear export
- GO:0051259 protein oligomerization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of MALT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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