MALT1 antibody

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Mouse anti MALT1

MALT1 antibody recognizes mucosa-associated lymphoid tissue lymphoma translocation protein 1, also known as MALT lymphoma-associated translocation or paracaspase. The antibody has been extensively validated for western blotting using whole cell lysates.

MALT1 Antibody with Control Lysate

MALT1 antibody recognizes mucosa-associated lymphoid tissue lymphoma translocation protein 1, also known as MALT lymphoma-associated translocation or paracaspase. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00038 WB 100 µl
VMA00038KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human MALT1 antibody recognizes human mucosal-associated lymphoid tissue lymphoma translocation 1 (MALT1), also known as MALT lymphoma-associated translocation and paracaspase. MALT1 is an 824 amino acid protein belonging to the peptidase C14B family containing a single death domain and two Ig-like C2-type domains. In normal lymphocytes, MALT1 plays an important role in antigen receptor-mediated lymphocyte activation. In T-cells MALT1 is recruited by activated CARMA1, along with Bcl-10, to form a CARMA1-Bcl10-MALT1 (CBM) complex which is involved in the activation of NF-kappaB (Yang et al. 2013).

MALT1 was initially identified as a recurrent translocation t(11;18)(q21:q21) in mucosal-associated lymphomas (Horsman et al. 1992), which creates a functional fusion oncoprotein consisting of MALT1 and apoptosis inhibitor API2 (Dierlamm et al. 1999). Studies suggest that overexpression of MALT1, or expression of the API2-MALT1 fusion protein, leads to the uncontrolled activation of NF-kappaB which is stimulus-independent (Zhou et al. 2005). Mouse anti Human MALT1 recognizes both MALT1 and the MALT1-API2 fusion protein (Rosebeck et al.2011, Murga Penas et al. 2007). The MALT1-API2 oncoprotein plays a role in non-canonical activation of NF-κb through NIK cleavage.

Mouse anti Human MALT1 antibody recognizes human MALT1 as a single band of ~92 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti MALT1 antibody detects a band of approximately 92 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 701-808 located within the C-terminus of human MALT1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UDY8 Related reagents
Entrez Gene
MALT1 Related reagents
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0002726 positive regulation of T cell cytokine production
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006508 proteolysis
GO:0006916 anti-apoptosis
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0032449 CBM complex
GO:0032743 positive regulation of interleukin-2 production
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043621 protein self-association
GO:0048471 perinuclear region of cytoplasm
GO:0050852 T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051168 nuclear export
GO:0051259 protein oligomerization
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of MALT1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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